Optimization of two-stage pretreatment for maximizing ethanol production in 1.5G technology
| Autor: | Herman Jan Pel, Vijay Singh, Ankita Juneja, Bert Noordam, Rahul Basu, Maaike M. Appeldoorn |
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| Rok vydání: | 2020 |
| Předmět: |
0106 biological sciences
Arabinose Technology Environmental Engineering Bioengineering Cellulase 010501 environmental sciences Xylose 01 natural sciences Zea mays chemistry.chemical_compound Hydrolysis 010608 biotechnology Ethanol fuel Food science Waste Management and Disposal 0105 earth and related environmental sciences Ethanol biology Renewable Energy Sustainability and the Environment General Medicine chemistry Fermentation biology.protein Stillage |
| Zdroj: | Bioresource technology. 320 |
| ISSN: | 1873-2976 |
| Popis: | Two-stage pretreatment conditions were optimized to convert corn fiber, separated from whole stillage in a corn dry grind ethanol plant, to fermentable sugars via hydrolysis. Liquid hot water pretreatment (25% solids) at 180 °C for 10 min, followed by three cycles of disk milling, provided maximum glucose, xylose, and arabinose yields of 88.5%, 41.0%, and 30.4% respectively after hydrolysis with Cellulase I. The glucose, xylose, and arabinose yields with Cellulase II at optimum conditions were 94.9%, 74.2%, and 66.3%, respectively. SSF of corn fiber using engineered yeast, with both Cellulase I and II, provided maximum ethanol concentrations of 2.13% and 2.73% (v/v). The protein content in the residual solid after fermentation was 47.95% and 52.05% for Cellulase I and II, respectively. This technology provides additional ethanol in a dry grind plant by converting corn fiber into ethanol and increases the protein content of DDGS, thereby improving the quality. |
| Databáze: | OpenAIRE |
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