Human napsin A: expression, immunochemical detection, and tissue localization
| Autor: | Dorothee Kling, Vesna Schauer-Vukasinovic, Fiona Grüninger, Thomas Giller, Daniel Bur |
|---|---|
| Rok vydání: | 1999 |
| Předmět: |
Napsin A antibody
Blotting Western Molecular Sequence Data Biophysics Cathepsin D Expression Spleen Kidney Biochemistry Antibodies Epitope Human kidney Human lung law.invention Epitopes Western blot Structural Biology law Genetics medicine Aspartic Acid Endopeptidases Humans Tissue Distribution Amino Acid Sequence Lung Molecular Biology Cells Cultured Sequence Homology Amino Acid biology medicine.diagnostic_test Human napsin A HEK 293 cells Cell Biology Immunohistochemistry Molecular biology medicine.anatomical_structure biology.protein Recombinant DNA Antibody |
| Zdroj: | FEBS Letters. 462:135-139 |
| ISSN: | 0014-5793 |
| DOI: | 10.1016/s0014-5793(99)01458-1 |
| Popis: | A novel aspartic proteinase, called napsin, has recently been found in human and mouse. Due to high similarity with cathepsin D a structural model of human napsin A could be built. Based on this model a potential epitope SFYLNRDPEEPDGGE has been identified, which was used to immunize rabbits. The resulting antibody was employed in monitoring the expression of recombinant human napsin A in HEK293 cell line. Western blot analysis confirmed the specificity of the antibody and showed that human napsin A is expressed as a single chain protein with the molecular weight of approximately 38 kDa. Immunohistochemical studies revealed high expression levels of napsin A in human kidney and lung but low expression in spleen. |
| Databáze: | OpenAIRE |
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