Dietary fish oil reduces oxidative DNA damage in rat colonocytes
| Autor: | Robert S. Chapkin, Raymond J. Carroll, Laura Bancroft, Stella S. Taddeo, Laurie A. Davidson, Joanne R. Lupton, Mary E. Murphy |
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| Rok vydání: | 2003 |
| Předmět: |
Male
medicine.medical_specialty Colon Drinking Apoptosis Inflammation Weight Gain medicine.disease_cause Biochemistry DNA Glycosylases Rats Sprague-Dawley DNA Adducts Eating Fish Oils Dietary Fats Unsaturated Physiology (medical) Internal medicine medicine Animals Anticarcinogenic Agents Oxoguanine glycosylase chemistry.chemical_classification Reactive oxygen species Fish oil Intestinal epithelium Diet Rats Oxidative Stress Endocrinology Gene Expression Regulation chemistry Colonic Neoplasms medicine.symptom Corn oil Oxidative stress DNA Damage |
| Zdroj: | Free Radical Biology and Medicine. 35:149-159 |
| ISSN: | 0891-5849 |
| DOI: | 10.1016/s0891-5849(03)00240-5 |
| Popis: | Prolonged generation of reactive oxygen species by inflammatory mediators can induce oxidative DNA damage (8-oxodG formation), potentially resulting in intestinal tumorigenesis. Fish oil (FO), compared to corn oil (CO), has been shown to downregulate inflammation and upregulate apoptosis targeted at damaged cells. We hypothesized FO could protect the intestine against 8-oxodG formation during dextran sodium sulfate- (DSS-) induced inflammation. We provided 60 rats with FO- or CO-supplemented diets for 2 weeks with or without 3% DSS in drinking water for 48 h. Half the treated rats received 48 additional h of untreated water before termination. Due to DSS treatment, the intestinal epithelium had higher levels of 8-oxodG (p =.04), induction of repair enzyme OGG1 mRNA (p =.02), and higher levels of apoptosis at the top of colonic crypts (p =.01) and in surface cells (p.0001). FO-fed rats, compared to CO, had lower levels of 8-oxodG (p =.05) and increased apoptosis (p =.04) in the upper crypt region; however, FO had no significant effect on OGG1 mRNA. We conclude that FO protects intestinal cells against oxidative DNA damage in part via deletion mechanisms. |
| Databáze: | OpenAIRE |
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