Methyl labeling of HeLa cell hnRNA: a comparison with mRNA
| Autor: | M. Morgan, Warren Jelinek, Y. Furuichi, M. Salditt-Georgieff, Aaron J. Shatkin, James E. Darnell |
|---|---|
| Rok vydání: | 1976 |
| Předmět: |
Cell Nucleus
chemistry.chemical_classification Messenger RNA Oligonucleotide RNA Methylation Biology Cleavage (embryo) Molecular biology General Biochemistry Genetics and Molecular Biology Mitochondria Cell nucleus medicine.anatomical_structure Biochemistry chemistry medicine Nucleotide RNA Messenger RNA Neoplasm Poly A Precursor mRNA HeLa Cells |
| Zdroj: | Cell. 7:227-237 |
| ISSN: | 0092-8674 |
| DOI: | 10.1016/0092-8674(76)90022-2 |
| Popis: | The majority of the mRNA molecules in HeLa cells contain 1-2 residue(s) of m6Ap and one blocked, methylated 5' terminal "cap" structure. The hnRNA, which is longer than mRNA, contains both m6Ap and caps but 4-6 times as many m6Ap residues per chain. In addition, nuclear molecules contain T2 RNA ase-resistant, methyl-labeled oligonucleotides ("di-" and "tri-" nucleotides) which are not found in mRNA. Some of the dinucleotides may be precursors to the 2'-0-methylated nucleotides in the cap structures. These results are compatible with internal methylation of hnRNA molecules (both m6Ap and 2'-0-methyl) followed by hnRNA cleavage and the addition of the cap structure to generate at least some of the HeLa cell mRNA. It also appears that some hnRNA molecules, which are longer than most mRNA molecules, contain cap structures suggesting the derivation of some mRNA molecules from the 5' regions of hnRNA. |
| Databáze: | OpenAIRE |
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