Co-carrying of KPC-2, NDM-5, CTX-M-3 and CTX-M-65 in three plasmids with serotype O89: H10 Escherichia coli strain belonging to the ST2 clone in China
| Autor: | Ying Li, Luhua Zhang, Xingyou Yang, Guangxi Wang, Li Fu, Shanmei Wang, Kelei Zhao, Yingshun Zhou, Zhikun Zhang, Xiangjing Yan |
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| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
clone (Java method) Serotype Male China Genotype Virulence Factors 030106 microbiology Virulence medicine.disease_cause Serogroup Microbiology Genome beta-Lactamases 03 medical and health sciences Plasmid Drug Resistance Multiple Bacterial medicine Escherichia coli Humans Gene Escherichia coli Infections Phylogeny Aged 80 and over biology Whole Genome Sequencing Escherichia coli Proteins Pneumonia biology.organism_classification Enterobacteriaceae Anti-Bacterial Agents 030104 developmental biology Infectious Diseases Carbapenem-Resistant Enterobacteriaceae Genes Bacterial Genome Bacterial Plasmids |
| Zdroj: | Microbial pathogenesis. 128 |
| ISSN: | 1096-1208 |
| Popis: | Carbapenem-resistant Enterobacteriaceae strains as a new serious threat for the public health have been increasingly reported worldwide. In this study, one multi-resistant Escherichia coli strain ZSH6 which co-carried blaKPC-2, blaNDM-5 and blaCTX-M, was isolated from human blood sample. By using plasmid conjugation experiments, ZSH6 was found to harbor three plasmids carrying the blaNDM-5 gene, the blaKPC-2 and blaCTX-M gene, respectively. Whole-genome sequencing of ZSH6 yielded 122 scaffolds of chromosomal DNA and three circular plasmids including pZSH6-blaKPC-2 (46,319 bp), pZSH6-blaNDM-5 (46,161bp) and pZSH6-blaCTX-M (184,723). The isolate was classified to Sequence Type 2 and to the O89: H10 serotype. The results of genome analyses revealed that ZSH6 carried three virulence factors (capU, gad and iss) and twenty resistance genes [blaKPC-2blaNDM-5, blaCTX-M-3, blaCTX-M-65, blaTEM-1, floR, tet(A), tet(B), dfrA17, aadA5, sul1, mdf(A), mph(A), erm(B), aph(3')-Ia, aph(3')-Ib, aph(4)-Ia, aph(6)-Id, aac(3)-Iva, aac(3)-IId]. Therefore, the co-existence of such a large number of resistance genes in multiple plasmids making ZSH6 highly resistant to almost all kinds of commonly used antibiotics, and brings a serious challenge for resistance control and clinical treatment of infections caused by this bacterium. |
| Databáze: | OpenAIRE |
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