Integrative analysis of transcriptome and metabolome reveals probiotic effects on cecal metabolism in broilers

Autor: Yanfei Wang, Xuan Liu, Hao Jia, Ruonan Zhang, Jiawei Guan, Lihuan Zhang
Rok vydání: 2023
Předmět:
Zdroj: Journal of the Science of Food and Agriculture. 103:2876-2888
ISSN: 1097-0010
0022-5142
Popis: Probiotics play an important role in the host and have attracted widespread attention as an alternative to antibiotics. Arbor Acres broilers were used in the present experiment and fed different doses of compound probiotics at 1, 5, and 10g kgWe discovered 425 differentially expressed genes (DEGs; upregulated: 256, downregulated: 169) in the cecal transcriptome study. These DEGs were assigned to fat metabolic pathways, such as the PPAR signaling pathway, according to KEGG analysis. Probiotics downregulated LPL and up-regulated PPARα expression in the cecum. In metabolome analysis of broilers cecum, we screened 86 differential metabolites and performed KEGG enrichment analysis of these metabolites. The KEGG analysis showed that these differentially expressed metabolites were annotated to nucleotide metabolism-related pathways, such as purine metabolism. In the cecum, probiotics upregulated the content of guanine, AMP, 3'-AMP, adenylosuccinate, deoxyguanosine, and ADP-ribose, whereas downregulated the content of 5-hydroxyisourate. Comprehensive transcriptome and metabolome analysis revealed that glycolysis, gluconeogenesis, and glycerophospholipid metabolism pathways were jointly enriched in cecum of broilers fed probiotics-containing diet.This study provides valuable information for studying the regulation and gene metabolism network of probiotics on caecal metabolism in broilers. This article is protected by copyright. All rights reserved.
Databáze: OpenAIRE
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