Estimation of the rate of 5-HT synthesis in the mouse brain by varius methods
| Autor: | Y. Morot-Gaudry, Sylvie Bourgoin, Michel Hamon, Jacques Glowinski, J. P. Ley |
|---|---|
| Rok vydání: | 1974 |
| Předmět: |
Serotonin
medicine.medical_specialty Monoamine Oxidase Inhibitors Carboxy-Lyases Stimulation Tritium 5-Hydroxytryptophan Mice chemistry.chemical_compound Pheniprazine Internal medicine Benzyl Compounds medicine Animals 5-HT receptor Brain Chemistry Pharmacology Analysis of Variance Probenecid Tryptophan Brain General Medicine Hydroxyindoleacetic Acid Chromatography Ion Exchange Pargyline Kinetics Hydrazines Spectrometry Fluorescence Endocrinology chemistry Female medicine.drug |
| Zdroj: | Naunyn-Schmiedeberg's Archives of Pharmacology. 282:223-238 |
| ISSN: | 1432-1912 0028-1298 |
| Popis: | Non-isotopic and isotopic methods were used to estimate the rate of 5-HT synthesis in the mouse brain. 5-HT and 5_HIAA levels were measured in tissues up to 10 min after the i. p. injection of pargyline (75 or 150 mg/kg) or pheniprazine (5 or 10 mg/kg) (5-HT and 5-HIAA, MAO-inhibitor methods). 5-HIAA levels were also estimated at various times after probenecid administration (200 or 400 mg/kg i. p.) (5-HIAA-probenecid method). 5-HTP levels were estimated at various times after the blockade of 5-HTP decarboxylase by Ro4-4602. (800, 1000 or 1200 mg/kg i. p.) (5-HTP method). Finally the rate of conversion of tryptophan into 5-HT has been estimated by measuring the initial accumulation of 3H-5-HT and 3H-5-HIAA in tissues following the intravenous injection of 3H-tryptophan (isotopic method). Rates of 5-HT synthesis obtained with the two MAO inhibitor methods were much higher (about 3 to 4 times) than those calculated with the 5-HTP and 5-HIAA-probenecid methods. An intermediate rate of 5-HT synthesis was found with the isotopic technique. The high rate of 5-HT synthesis observed with the 5-HT, MAO inhibitor method (about 8 nmoles/g/h) was not related to a stimulation of 5-HT synthesis. Differences seen with all methods are discussed with respect to results obtained by various groups of workers. |
| Databáze: | OpenAIRE |
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