Methanocalculus taiwanensis sp. nov., isolated from an estuarine environment
Autor: | Ming-Shing Chiou, Chia-Chi Wang, Mei-Chin Lai, Li-Jane Lai, Chin-Ming Shu, Chia-Chi Liu, Ming-Jen Chuang, Sheng-Chung Chen, Jack Jay Hua, Tong-Yung Hong |
---|---|
Rok vydání: | 2002 |
Předmět: |
Methanocalculus
Methanogenesis Archaeal Proteins Molecular Sequence Data Taiwan RNA Archaeal Methanocalculus halotolerans DNA Ribosomal Microbiology Methanomicrobiales Genes Archaeal Species Specificity RNA Ribosomal 16S Terminology as Topic Phylogeny Ecology Evolution Behavior and Systematics Base Sequence biology General Medicine Ribosomal RNA 16S ribosomal RNA biology.organism_classification Methanogen Drug Resistance Multiple Microscopy Electron DNA Archaeal Biochemistry Water Microbiology Bacteria |
Zdroj: | International Journal of Systematic and Evolutionary Microbiology. 52:1799-1806 |
ISSN: | 1466-5034 1466-5026 |
Popis: | Two novel hydrogenotrophic methanogens, designated strains P2F9704aT and P2F9705, were isolated from an estuary in Eriln Shi, Taiwan. The cells of strain P2F9704aT were non-motile, irregular cocci 0.9-1.4 microm in diameter. They stained gram-negative. The cells catabolized formate and H2+CO2 to produce methane, but did not utilize acetate, methanol, trimethylamine, ethanol or secondary alcohols as methanogenic substrates. The optimal growth parameters for strain P2F9704aT were pH 6.7, 37 degrees C and 0.5% NaCl. Acetate was required for cell growth even though it was not a substrate for methanogenesis. The trace element tungsten was not required but slightly stimulated the growth of strain P2F9704aT. However, tungsten extended the growth ranges relating to temperature, pH and salt. The sequences of the 16S rRNA genes of strains P2F9704aT and P2F9705 were nearly identical and possessed 99.1 and 98.5% similarity to the genes of Methanocalculus pumilus and Methanocalculus halotolerans, respectively. In addition, strain P2F9704aT possessed 14 and 12% DNA relatedness with respect to Methanocalculus pumilus and Methanocalculus halotolerans, respectively. In addition, the optimal salt concentrations, the cellular protein profiles and the molecular masses of surface-layer protein subunits of strain P2F9704aT were different from those of the other two known Methanocalculus species. On the basis of these observations, it is proposed that these two organisms should be placed in a new species, namely Methanocalculus taiwanensis. The type strain is P2F9704aT (= OCM 671T = CCRC 16182T = DSM 14663T). |
Databáze: | OpenAIRE |
Externí odkaz: |