Prostaglandin-E 1 has a protective effect on renal ischemia/reperfusion-induced oxidative stress and inflammation mediated gastric damage in rats

Autor: Selda Gezginci-Oktayoglu, Nurcan Orhan, Sehnaz Bolkent
Rok vydání: 2016
Předmět:
Male
0301 basic medicine
medicine.medical_specialty
Interleukin-1beta
Immunology
Anti-Inflammatory Agents
Inflammation
Kidney
urologic and male genital diseases
medicine.disease_cause
03 medical and health sciences
chemistry.chemical_compound
Postoperative Complications
0302 clinical medicine
Internal medicine
medicine
Animals
Humans
Immunology and Allergy
Alprostadil
Pharmacology
L-Lactate Dehydrogenase
Renal ischemia
Tumor Necrosis Factor-alpha
business.industry
Stomach
NF-kappa B
medicine.disease
Malondialdehyde
Kidney Transplantation
Rats
Oxidative Stress
Mononuclear cell infiltration
030104 developmental biology
medicine.anatomical_structure
Endocrinology
chemistry
Gastritis
Reperfusion Injury
030220 oncology & carcinogenesis
lipids (amino acids
peptides
and proteins)
Tumor necrosis factor alpha
medicine.symptom
Reactive Oxygen Species
business
Reperfusion injury
Oxidative stress
Zdroj: International Immunopharmacology. 36:142-150
ISSN: 1567-5769
Popis: Gastrointestinal complications are frequent in renal transplant recipients. In this regard, renal ischemia/reperfusion injury (IRI)-induced gastric damage seems to be important and there is no data available on the mechanism of this pathology. Because of its anti-inflammatory and anti-oxidant properties, it can be suggested that prostaglandin-E1 (PGE1) protects cells from renal IRI-induced gastric damage. The aim of this study was to investigate the molecular mechanisms of gastric damage induced by renal IRI and the effect of PGE1 on these mechanisms. We set an experiment with four different animal groups: physiological saline-injected and sham-operated rats, PGE1 (20μg/kg)-administered and sham operated rats, renal IRI subjected rats, and PGE1-administered and renal IRI subjected rats. The protective effect of PGE1 on renal IRI-induced gastric damage was determined based on reduced histological damage and lactate dehydrogenase activity. Moreover, we demonstrated that PGE1 shows its protective effect through reducing the production of reactive oxygen species and malondialdehyde levels. During histological examination, we observed the presence of common mononuclear cell infiltration. Therefore, pro-inflammatory cytokines tumor necrosis factor-α and interleukin-1β levels were measured and it has been shown that PGE1 suppressed both cytokines. Furthermore, it was found that PGE1 reduced the number of NF-κB(+) and caspase-3(+) inflammatory cells, and also NF-κB DNA-binding activity, while increasing proliferating cell nuclear antigen(+) epithelial cells in the stomach tissue of rats subjected to renal IR. Our data showed that PGE1 has a protective effect on renal IRI-induced oxidative stress and inflammation mediated gastric damage in rats.
Databáze: OpenAIRE
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