Regulation of Protein Mobility in Cell Membranes: A Dynamic Corral Model
| Autor: | Frank L. H. Brown, Kent R. Wilson, David M. Leitner |
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| Rok vydání: | 2000 |
| Předmět: |
Models
Statistical biology Chemistry Monte Carlo method Cell Membrane Biophysics Membrane Proteins Gating Models Biological Transport protein Cell membrane Kinetics Membrane medicine.anatomical_structure biology.protein medicine Statistical physics Diffusion (business) Cytoskeleton Band 3 Monte Carlo Method Research Article Probability |
| Zdroj: | Biophysical Journal. 78(1):125-135 |
| ISSN: | 0006-3495 |
| DOI: | 10.1016/s0006-3495(00)76579-9 |
| Popis: | We analyze a two-state stochastic corral model for regulation of protein diffusion in a cell membrane. This model could mimic control of protein transport in the membrane by the cytoskeleton. The dynamic corral acts as a gate which when open permits an otherwise trapped protein to escape to a neighboring corral in the cytoskeletal network. We solve for the escape rate over a wide range of parameters of the model, and compare these results with Monte Carlo simulations. Upon introducing measured values of the model parameters for Band 3 in erythrocyte membranes, we are able to estimate the value for one unknown parameter, the average rate at which the corral closes. The ratio of calculated closing rate to measured opening rate is roughly 100:1, consistent with a gating mechanism whereby protein mobility is regulated by dissociation and reassociation of segments of the cytoskeletal network. |
| Databáze: | OpenAIRE |
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