New Promising Targets for Synthetic Omptin-Based Peptide Vaccine against Gram-Negative Pathogens
| Autor: | Onega V. Ulianova, Valentina A. Feodorova, Vladimir L. Motin, Maria A. Khizhnyakova, Sergey S Ulyanov, Anna Lyapina, Sergey S. Zaitsev, L. V. Sayapina |
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| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
Proteases Omptin 030106 microbiology Immunology lcsh:Medicine Article Epitope Microbiology 03 medical and health sciences Shigella flexneri omptin family proteases Drug Discovery peptide vaccine Pharmacology (medical) Pharmacology Gram-negative pathogens biology lcsh:R B-cell epitope biology.organism_classification OmpT humanities 030104 developmental biology Infectious Diseases vaccine development Yersinia pestis Salmonella enterica Peptide vaccine multi-pathogen vaccine broad-spectrum vaccine peptide ELISA |
| Zdroj: | Vaccines, Vol 7, Iss 2, p 36 (2019) Vaccines Volume 7 Issue 2 |
| ISSN: | 2076-393X |
| Popis: | Omptins represent a family of proteases commonly found in various Gram-negative pathogens. These proteins play an important role in host&ndash pathogen interaction and have been recognized as key virulence factors, highlighting the possibility of developing an omptin-based broad-spectrum vaccine. The prototypical omptin, His-tagged recombinant Pla, was used as a model target antigen. In total, 46 linear and 24 conformational epitopes for the omptin family were predicted by the use of ElliPro service. Among these we selected highly conserved, antigenic, non-allergenic, and immunogenic B-cell epitopes. Five epitopes (2, 6, 8, 10, and 11 corresponding to Pla regions 52&ndash 60, 146&ndash 150, 231&ndash 234, 286&ndash 295, and 306&ndash 311, respectively) could be the first choice for the development of the new generation of target-peptide-based vaccine against plague. The partial residues of omptin epitopes 6, 8, and 10 (regions 136&ndash 145, 227&ndash 230, and 274&ndash 285) could be promising targets for the multi-pathogen vaccine against a group of enterobacterial infections. The comparative analysis and 3D modeling of amino acid sequences of several omptin family proteases, such as Pla (Yersinia pestis), PgtE (Salmonella enterica), SopA (Shigella flexneri), OmpT, and OmpP (Escherichia coli), confirmed their high cross-homology with respect to the identified epitope clusters and possible involvement of individual epitopes in host&ndash pathogen interaction. |
| Databáze: | OpenAIRE |
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