Association between IL-6 production in synovial explants from rheumatoid arthritis patients and clinical and imaging response to biologic treatment: A pilot study

Autor: M. Andersen, Niels Henrik Søe, Ulrik Morch, Bente Danneskiold-Samsøe, Søren Torp-Pedersen, Mikael Boesen, Else Marie Bartels, Kalle Söderström, Pieter Spee, Karen Ellegaard, Henning Bliddal, Lars Karlsson
Rok vydání: 2018
Předmět:
Male
0301 basic medicine
Image Processing
lcsh:Medicine
Antirheumatic Agents/therapeutic use
Pilot Projects
Gastroenterology
Arthroscopic procedure
Diagnostic Radiology
Arthritis
Rheumatoid
Tissue Culture Techniques
Explant Cultures
0302 clinical medicine
Ultrasound Imaging
Image Processing
Computer-Assisted
Medicine and Health Sciences
Medicine
Ultrasonography
Doppler
Color
lcsh:Science
Musculoskeletal System
Chemokine CCL2
Multidisciplinary
biology
medicine.diagnostic_test
Pharmaceutics
Radiology and Imaging
Synovial Membrane
Middle Aged
Wrist
Prognosis
Magnetic Resonance Imaging
Interleukin-6/analysis
Arms
Antirheumatic Agents
Rheumatoid arthritis
Synovial Membrane/diagnostic imaging
Engineering and Technology
Female
Biological Cultures
Anatomy
Research Article
Adult
Treatment response
medicine.medical_specialty
Image Processing
Computer-Assisted/methods
Imaging Techniques
Immunology
Rheumatoid Arthritis
In Vitro Techniques
Biologic treatment
Research and Analysis Methods
Autoimmune Diseases
03 medical and health sciences
Pharmacotherapy
Rheumatology
Drug Therapy
Diagnostic Medicine
Internal medicine
Humans
Interleukin 6
Aged
030203 arthritis & rheumatology
Interleukin-6
business.industry
Arthritis
Limbs (Anatomy)
lcsh:R
Biology and Life Sciences
Ultrasonography
Doppler
Color/methods
Magnetic resonance imaging
medicine.disease
Joints (Anatomy)
030104 developmental biology
Chemokine CCL2/metabolism
Case-Control Studies
Signal Processing
biology.protein
Clinical Immunology
lcsh:Q
Clinical Medicine
business
Arthritis
Rheumatoid/diagnostic imaging
Explant culture
Zdroj: PLoS ONE, Vol 13, Iss 5, p e0197001 (2018)
PLoS ONE
Andersen, M, Boesen, M, Ellegaard, K, Söderström, K, Søe, N H, Spee, P, Mørch, U G W, Torp-Pedersen, S, Bartels, E M, Danneskiold-Samsøe, B, Karlsson, L & Bliddal, H 2018, ' Association between IL-6 production in synovial explants from rheumatoid arthritis patients and clinical and imaging response to biologic treatment : A pilot study ', PLoS ONE, vol. 13, no. 5, e0197001 . https://doi.org/10.1371/journal.pone.0197001
ISSN: 1932-6203
DOI: 10.1371/journal.pone.0197001
Popis: INTRODUCTION: The need for biomarkers which can predict disease course and treatment response in rheumatoid arthritis (RA) is evident. We explored whether clinical and imaging responses to biologic disease modifying anti-rheumatic drug treatment (bDMARD) were associated with the individual's mediator production in explants obtained at baseline.METHODS: RA Patients were evaluated by disease activity score 28 joint C-reactive protein (DAS 28-)), colour Doppler ultrasound (CDUS) and 3 Tesla RA magnetic resonance imaging scores (RAMRIS). Explants were established from synovectomies from a needle arthroscopic procedure prior to initiation of bDMARD. Explants were incubated with the bDMARD in question, and the productions of interleukin-6 (IL-6), monocyte chemo-attractive protein-1 (MCP-1) and macrophage inflammatory protein-1-beta (MIP-1b) were measured by multiplex immunoassays. The changes in clinical and imaging variables following a minimum of 3 months bDMARD treatment were compared to the baseline explant results. Mixed models and Spearman's rank correlations were performed. P-values below 0.05 were considered statistically significant.RESULTS: 16 patients were included. IL-6 production in bDMARD-treated explants was significantly higher among clinical non-responders compared to responders (P = 0.04), and a lack of suppression of IL-6 by the bDMARDS correlated to a high DAS-28 (ρ = 0.57, P = 0.03), CDUS (ρ = 0.53, P = 0.04) and bone marrow oedema (ρ = 0.56, P = 0.03) at follow-up. No clinical association was found with explant MCP-1 production. MIP-1b could not be assessed due to a large number of samples below the detection limit.CONCLUSIONS: Synovial explants appear to deliver a disease-relevant output testing which when carried out in advance of bDMARD treatment can potentially pave the road for a more patient tailored treatment approach with better treatment effects.
Databáze: OpenAIRE
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