Functional NMDA Receptors at Axonal Growth Cones of Young Hippocampal Neurons
| Autor: | Robert J. Wenthold, Philip Y. Wang, Stephan D. Brenowitz, Ronald S. Petralia, Ya-Xian Wang |
|---|---|
| Rok vydání: | 2011 |
| Předmět: |
Nervous system
Growth Cones Hippocampus chemistry.chemical_element Cell Enlargement Biology Hippocampal formation Calcium Receptors N-Methyl-D-Aspartate Article Calcium in biology Calcium imaging medicine Animals Growth cone Cells Cultured Neurons Voltage-dependent calcium channel musculoskeletal neural and ocular physiology General Neuroscience Axons Rats medicine.anatomical_structure nervous system chemistry sense organs Neuroscience |
| Zdroj: | Journal of Neuroscience. 31:9289-9297 |
| ISSN: | 1529-2401 0270-6474 |
| DOI: | 10.1523/jneurosci.5639-10.2011 |
| Popis: | N-methyl d-aspartate receptors (NMDARs) are critical to the development of the nervous system, although their roles at axonal growth cones are unclear. We examined NMDAR localization and function at axonal growth cones of young hippocampal neurons. Our immunocytochemical data showed that native and transfected NMDAR subunits are expressed in axons and growth cones of young (days in vitro 3–6, DIV3-6) hippocampal rat neurons. Moreover, immunogold electron microscopy showed that NR1 is expressed in growth cones of postnatal day 2 (P2) rat hippocampus. Local application of NMDAR agonists to growth cones of voltage clamped neurons evoked inward currents that were blocked by bath application of an NMDAR antagonist (DL-APV), indicating that these NMDARs are functional. In addition, calcium imaging experiments indicated that NMDARs present in growth cones mediate calcium influx. Calcium transients in growth cones persisted despite pharmacological blockade of voltage sensitive calcium channels and depletion of intracellular calcium stores. Our findings reveal the presence of functional NMDARs in axons and growth cones of young neurons, suggesting a role for these receptors in axonal guidance and synapse formation during neuronal development. |
| Databáze: | OpenAIRE |
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