Onlay bone augmentation on mouse calvarial bone using a hydroxyapatite/collagen composite material with total blood or platelet-rich plasma
| Autor: | Shinpei Matsuda, Kazuo Sano, Hitoshi Yoshimura, Mayumi Umebayashi, Hisato Yoshida, Izumi Asahina, Yoshinori Sumita, Hideki Kimura, Seigo Ohba |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
Male
Artificial bone medicine.medical_treatment H&E stain Mice Inbred Strains 02 engineering and technology Sodium Chloride Mice 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Osteogenesis Hyaluronic acid medicine Animals Humans Hyaluronic Acid Composite material General Dentistry Saline Platelet-Rich Plasma Skull 030206 dentistry Cell Biology General Medicine 021001 nanoscience & nanotechnology Transplantation Blood Durapatite medicine.anatomical_structure Otorhinolaryngology chemistry Platelet-rich plasma Bone Substitutes Female Collagen Bone marrow 0210 nano-technology |
| Zdroj: | Archives of Oral Biology. 61:23-27 |
| ISSN: | 0003-9969 |
| DOI: | 10.1016/j.archoralbio.2015.10.012 |
| Popis: | Objective The aim of this study was to assess newly formed onlay bone on mouse calvarial bone using a new artificial bone material, a hydroxyapatite/collagen composite, with total blood or platelet-rich plasma. Design The hydroxyapatite/collagen composite material with normal saline, total blood or platelet-rich plasma was transplanted on mouse calvarial bone. The mice were sacrificed and the specimens were harvested four weeks after surgery. The newly formed bone area was measured on hematoxylin and eosin stained specimens using Image J software. Results The hydroxyapatite/collagen composite materials with total blood or platelet-rich plasma induced a significantly greater amount of newly formed bone than that with normal saline. Moreover, bone marrow was observed four weeks after surgery in the transplanted materials with total blood or platelet-rich plasma but not with normal saline. However, there were no significant differences in the amount of newly formed bone between materials used with total blood versus platelet-rich plasma. Conclusions The hydroxyapatite/collagen composite material was valid for onlay bone augmentation and this material should be soaked in total blood or platelet-rich plasma prior to transplantation. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect