Comparison of real‐time and droplet digital PCR to detect and quantify SARS‐CoV‐2 RNA in plasma
| Autor: | José María Eiros, David J. Kelvin, Rosario Menéndez, Natalia Blanca-López, Ferran Barbé, Marta Domínguez-Gil, Dariela Micheloud, Antoni Torres, Ruth Oneizat, Ali Toloue Ostadgavahi, Jesus F. Bermejo-Martin, Felipe Pérez-García, Wysali Trapiello, Carolina Puertas, Elena Bustamante, Alyson A. Kelvin, Raquel Almansa, Raúl Méndez, Cristina Doncel, Milagros González-Rivera, Ana P. Tedim, Pablo Ryan, Ricard Ferrer, José Manuel Gómez, Ryan Booth |
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| Rok vydání: | 2021 |
| Předmět: |
Male
viruses Clinical Biochemistry ddPCR 030204 cardiovascular system & hematology Polymerase Chain Reaction Severity of Illness Index Biochemistry Gastroenterology SARS‐CoV‐2 0302 clinical medicine Ambulatory Care Medicine Statistical analysis Digital polymerase chain reaction 030212 general & internal medicine skin and connective tissue diseases General Medicine Middle Aged 3. Good health Intensive Care Units RNAemia RNA Viral Female Original Article RT‐qPCR medicine.medical_specialty Coronavirus disease 2019 (COVID-19) Concordance Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) COVID-19 RNAemia RT-qPCR SARS-CoV-2 ddPCR viral RNA load Real-Time Polymerase Chain Reaction 03 medical and health sciences Disease severity COVID‐19 Internal medicine Patients' Rooms Humans Viral rna Aged SARS-CoV-2 business.industry fungi COVID-19 RNA Original Articles respiratory tract diseases body regions business viral RNA load |
| Zdroj: | EUROPEAN JOURNAL OF CLINICAL INVESTIGATION r-IIS La Fe. Repositorio Institucional de Producción Científica del Instituto de Investigación Sanitaria La Fe instname European Journal of Clinical Investigation |
| ISSN: | 1365-2362 0014-2972 |
| DOI: | 10.1111/eci.13501 |
| Popis: | Background The presence of SARS‐CoV‐2 RNA in plasma has been linked to disease severity and mortality. We compared RT‐qPCR to droplet digital PCR (ddPCR) to detect SARS‐CoV‐2 RNA in plasma from COVID‐19 patients (mild, moderate, and critical disease). Methods The presence/concentration of SARS‐CoV‐2 RNA in plasma was compared in three groups of COVID‐19 patients (30 outpatients, 30 ward patients and 30 ICU patients) using both RT‐qPCR and ddPCR. Plasma was obtained in the first 24h following admission, and RNA was extracted using eMAG. ddPCR was performed using Bio‐Rad SARS‐CoV‐2 detection kit, and RT‐qPCR was performed using GeneFinder™ COVID‐19 Plus RealAmp Kit. Statistical analysis was performed using Statistical Package for the Social Science. Results SARS‐CoV‐2 RNA was detected, using ddPCR and RT‐qPCR, in 91% and 87% of ICU patients, 27% and 23% of ward patients and 3% and 3% of outpatients. The concordance of the results obtained by both methods was excellent (Cohen's kappa index = 0.953). RT‐qPCR was able to detect 34/36 (94.4%) patients positive for viral RNA in plasma by ddPCR. Viral RNA load was higher in ICU patients compared with the other groups (P 85%). RT‐qPCR was as useful as ddPCR to detect and quantify SARS‐CoV‐2 RNAemia in plasma. |
| Databáze: | OpenAIRE |
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