High-sensitive chemiluminescent assay for cholesterol
| Autor: | Jean-Pierre Ternaux, Huguette Lafont, Marie-Claude Chamoin, Monique Charbonnier |
|---|---|
| Rok vydání: | 1994 |
| Předmět: |
Cholesterol oxidase
Biophysics Biochemistry Sensitivity and Specificity Luminol law.invention chemistry.chemical_compound Endocrinology law Blood plasma Animals Sodium Cholate Rats Wistar Chemiluminescence Cerebral Cortex Chromatography biology Cholesterol Hydrogen-Ion Concentration Rats chemistry Solubility Luminescent Measurements biology.protein lipids (amino acids peptides and proteins) Quantitative analysis (chemistry) Peroxidase |
| Zdroj: | Biochimica et biophysica acta. 1210(2) |
| ISSN: | 0006-3002 |
| Popis: | Chemiluminescent measurement of cholesterol can be performed in various biological tissues and fluids. The method described in this study has a sensitivity of 54 pmol. The tissue samples used for the determination of cholesterol can be reduced to as little as 1 mg and assay can be performed on diluted biological fluids, allowing sampling of plasma or serum as little as 5 μl. Cholesterol is solubilized in sodium cholate and aliquots are added to a reaction mixture containing cholesterol oxidase, luminol and peroxidase. Cholesterol oxidase, in the presence of cholesterol yields H2O2 which produces light in presence of luminol and peroxidase. Emitted light is quantified at a wavelength of 420 nm by means of a photomultiplier. Optimal conditions of the assay were determined and examples of cholesterol determinations, in blood plasma and nervous tissues, are presented. |
| Databáze: | OpenAIRE |
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