Identification of distinct regions of allelic loss on chromosome 13q in nasopharyngeal cancer from paraffin embedded tissues
| Autor: | Sairoong Sakdikul, Narin Voravud, Shanop Shuangshoti, Virote Sriuranpong, Apiwat Mutirangura, Pakpoom Supiyaphun, Rattana Chatsantikul, Wichai Pornthanakasem, Navapun Charuruks |
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| Rok vydání: | 1999 |
| Předmět: |
Adult
Male Cancer Research medicine.medical_specialty Tumor suppressor gene Loss of Heterozygosity Locus (genetics) Biology Retinoblastoma Protein law.invention Loss of heterozygosity law medicine Humans Deletion mapping Alleles Polymerase chain reaction Aged Chromosome 13 Aged 80 and over Paraffin Embedding Chromosomes Human Pair 13 Cytogenetics Chromosome Mapping Nasopharyngeal Neoplasms DNA Neoplasm Middle Aged Molecular biology Oncology Cancer research Microsatellite Female Microsatellite Repeats |
| Zdroj: | International Journal of Cancer. 83:210-214 |
| ISSN: | 1097-0215 0020-7136 |
| Popis: | Our main purpose was to identify tumor suppressor gene loci on chromosome 13 responsible for nasopharyngeal cancer (NPC) development by analyzing loss of heterozygosity (LOH) and RB protein expression in paraffin embedded tissues. Normal and tumor DNA were extracted from microdissected samples, and their whole genomes were amplified using degenerate oligonucleotide primers. The polymerase chain reaction (PCR) products were analyzed by repeated amplification using primers derived from 16 microsatellite regions spanning the long arm of this chromosome. Among 50 informative cases, LOH was observed in 44 tumors. Thirty-one tumors displayed partial loss and provided an informative basis for detailed deletion mapping. Three minimal regions of loss were delineated; the first flanked by D13S120 and D13S219, the second by D13S126 and D13S119, and the third by D13S137 and 13qter. These 3 regions were linked to BRCA2 on 13q12, RB1 on 13q14, and 13q14.3-ter, respectively. Seven and 4 cases showed LOH either on 13q12 or 13q14, respectively. Nineteen cases showed LOH of both loci separately. One NPC displayed 13q12 and 13q14.3-ter LOH. RB protein expression was detectable in 76% of the cases. Ten out of 15 cases with the allelic losses limited to 13q14 showed RB protein expression. Contrasting that, 6 out of 7 cases devoid of RB protein expressions showed 13q14LOH. In conclusion, 13qLOH, involving 3 tumor suppressor gene loci, appears to be a frequent genetic event occurring during NPC development. However, other tumor suppressor genes besides RB1, may be responsible for the majority of 13q14LOH. Int. J. Cancer 83:210–214, 1999. © 1999 Wiley-Liss, Inc. |
| Databáze: | OpenAIRE |
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