Hydroethanolic extract of Juglans regia L. green husks: A source of bioactive phytochemicals
| Autor: | Olga Ferreira, Ricardo C. Calhelha, Maria José Alves, João A. P. Coutinho, Lillian Barros, Isabel C.F.R. Ferreira, Vanessa Pedrosa Vieira, Carla Pereira, Rui M.V. Abreu |
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| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
Antioxidant
Swine Thiobarbituric acid medicine.medical_treatment Cytotoxicity Phytochemicals Anti-Inflammatory Agents Juglans regia L. husks Toxicology Antioxidants chemistry.chemical_compound Flavonols Tetralone Nuts Food science chemistry.chemical_classification 0303 health sciences biology 04 agricultural and veterinary sciences General Medicine Haemolysis 040401 food science Anti-Bacterial Agents Antibacterial Cytotoxicity Juglans medicine.drug_class Microbial Sensitivity Tests Anti-inflammatory 03 medical and health sciences 0404 agricultural biotechnology Phenols Cell Line Tumor medicine TBARS Animals Humans Tetralones 030304 developmental biology Flavonoids Bacteria Plant Extracts biology.organism_classification Naphthalene derivatives Phenolic compounds Antibacterial chemistry Drug Screening Assays Antitumor Food Science |
| Zdroj: | Repositório Científico de Acesso Aberto de Portugal Repositório Científico de Acesso Aberto de Portugal (RCAAP) instacron:RCAAP |
| Popis: | Juglans regia L. (walnut) green husks are an important fraction of waste resulting from the walnut production, thus representing an interesting natural matrix to explore as a source of bioactive compounds. In this work, the hydroethanolic extract of walnut green husks was studied considering the phytochemical composition and the biological activity using different cell model assays, most of them evaluated for the first time for this matrix. From the HPLC-DAD-ESI/MSn analysis, sixteen compounds were identified, being the extract mostly composed of naphthalene derivatives (including tetralone derivatives) and less abundant in phenolic compounds (hydroxycinnamic acids and flavonols). The cytotoxic potential of the extract was assessed against tumour (MCF- 7, NCI–H460, HeLa and HepG2) and non-tumour (PLP2) cell lines. Moreover, the antioxidant activity of the extract was evaluated by inhibition of the oxidative haemolysis (OxHLIA) and the formation of thiobarbituric acid reactive substances (TBARS), and the anti-inflammatory potential by the inhibition of the NO production by the RAW264.7 cell culture. The antibacterial effects of the extract were also evaluated against Gram-negative and Gram-positive bacteria. The results obtained represent a stepping stone for the development of future applications using walnut green husks as a source of added value compounds with bioactive potential. The authors thank the Foundation for Science and Technology (FCT, Portugal) and FEDER under Programme PT2020 for financial support to CIMO (UID/AGR/00690/2019) and V. Vieira grant (SFRH/BD/ 108487/2015); national funding by FCT, P.I., through the institutional scientific employment program-contract for L. Barros and R. Calhelha contracts, and C. Pereira contract celebrated though the program-contract foreseen in No. 4, 5 and 6 of article 23° of Decree-Law No. 57/ 2016, of 29th August, amended by Law No. 57/2017, of 19th July. To UID/CTM/50011/2019 (CICECO), financed by national funds through the FCT/MCTES. To POCI-01-0145-FEDER-006984 (LA LSRE-LCM), funded by ERDF, through POCI-COMPETE2020 and FCT. To project AllNat - POCI-01-0145-FEDER-030463 (PTDC/EQU-EPQ/30463/ 2017), funded by FEDER funds through COMPETE2020 – Programa Operacional Competitividade e Internacionalização (POCI), and by national funds through Foundation for Science and Technology (FCT/ MCTES), and to FEDER-Interreg España-Portugal programme for financial support through the project 0377_Iberphenol_6_E. info:eu-repo/semantics/publishedVersion |
| Databáze: | OpenAIRE |
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