Outbreak analysis and typing of MRSA isolates by automated repetitive-sequence-based PCR in a region with multiple strain types causing epidemics
| Autor: | Päivi Tissari, S.-S. Kaukoranta, J. J. Hirvonen, Tanja Pasanen, Jaana Vuopio, Saara Salmenlinna |
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| Rok vydání: | 2012 |
| Předmět: |
Microbiology (medical)
Adult Methicillin-Resistant Staphylococcus aureus medicine.medical_specialty Repetitive Sequences Multiple strain Biology medicine.disease_cause Polymerase Chain Reaction law.invention Disease Outbreaks Medical microbiology law Pulsed-field gel electrophoresis medicine Cluster Analysis Humans Typing Polymerase chain reaction Aged Repetitive Sequences Nucleic Acid Automation Laboratory Molecular Epidemiology Outbreak General Medicine biochemical phenomena metabolism and nutrition Staphylococcal Infections bacterial infections and mycoses Virology Molecular Typing Infectious Diseases Staphylococcus aureus |
| Zdroj: | European journal of clinical microbiologyinfectious diseases : official publication of the European Society of Clinical Microbiology. 31(11) |
| ISSN: | 1435-4373 |
| Popis: | The usefulness and performance of repetitive-sequence-based polymerase chain reaction (rep-PCR), the DiversiLab system, in the epidemiological surveillance for methicillin-resistant Staphylococcus aureus (MRSA) strain typing was assessed. MRSA isolates from five distinct outbreaks with precise epidemiological data (n = 69) and from the culture collection of well-characterized MRSA strains (n = 132) consisting of 35 spa and 23 pulsed-field gel electrophoresis (PFGE) types were analyzed. The typing results of the DiversiLab system in outbreak analysis were compared to the spa and PFGE typing methods. The DiversiLab system proved to be a reliable tool for the rapid first-line typing of MRSA isolates, showing a good reliability in distinguishing MRSA strains in an area where several MRSA types were causing epidemics. This, however, required that the automatic clustering was combined with manual interpretation using the pattern overlay function when the strain types showing high similarity were clustered together. All outbreaks were distinguished with the DiversiLab system and the PFGE method, but not with the spa typing method. The overall discriminatory power of the DiversiLab system in differentiating diverse MRSA strains proved to be good. We also demonstrated that, in addition to the genetic relatedness analysis of MRSA strains, it is important to obtain accurate epidemiological information in order to perform reliable epidemiological surveillance studies. |
| Databáze: | OpenAIRE |
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