cDNA Cloning of a Mannose-Binding Lectin-Associated Serine Protease (MASP) Gene from Hagfish (Eptatretus burgeri)
| Autor: | Tamotsu Fujii, Yoshiaki Sugawara, Liqiu Song, Kazufumi Takamune |
|---|---|
| Rok vydání: | 2005 |
| Předmět: |
endocrine system
DNA Complementary Molecular Sequence Data Complementary DNA biology.animal Eptatretus burgeri Animals Cluster Analysis Amino Acid Sequence Cloning Molecular Complement Activation Phylogeny DNA Primers Mannan-binding lectin Serine protease Base Sequence biology Reverse Transcriptase Polymerase Chain Reaction cDNA library Sequence Analysis DNA Blotting Northern biology.organism_classification Molecular biology C3-convertase Gene Components Mannose-Binding Protein-Associated Serine Proteases Lectin pathway biology.protein Hagfishes Animal Science and Zoology Hagfish |
| Zdroj: | Zoological Science. 22:897-904 |
| ISSN: | 0289-0003 |
| DOI: | 10.2108/zsj.22.897 |
| Popis: | Hagfish, agnathan cyclostome, is the most primitive extant vertebrate and its complement (C) system seems to be a primordial system in comparison with a well-developed C system in gnathostome vertebrates. From a phylogenic perspective of defense mechanisms, we have isolated complement C3 from the serum of hagfish (Eptatretus burgeri). In this study, we first attempted to identify a hagfish Bf or C2 as a C3 convertase by RT-PCR using degenerative primers designed on the basis of the conserved amino acid stretches among the several kinds of serine proteases. Contrary to our expectation, homology search of cloned RT-PCR product suggested that there was a partial cDNA encoding the homologue of neither Bf nor C2 but a mannose-binding lectin-associated serine protease (MASP). Analyses of a full-length cDNA clone isolated from a hagfish liver cDNA library by using the partial cDNA as a probe indicated that this cDNA encoded hagfish MASP 1. This evidence strongly suggests that the hagfish defends itself against pathogens at least by the complement system composed of lectin pathway. |
| Databáze: | OpenAIRE |
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