Light-induced formation of dimeric LHCII
| Autor: | Karol Sowinski, Ewa Janik, Monika Zubik, Wiesław I. Gruszecki, Wojciech Grudzinski, Joanna Bednarska, Rafal Luchowski |
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| Rok vydání: | 2017 |
| Předmět: |
Chlorophyll
0106 biological sciences 0301 basic medicine Light Dimer Light-Harvesting Protein Complexes Plant Science Thylakoids 01 natural sciences Biochemistry Protein Structure Secondary Dissociation (chemistry) HIGHER-PLANTS chemistry.chemical_compound Spinacia oleracea Photosynthesis General Medicine FLUORESCENCE CORRELATION SPECTROSCOPY Monomer ENERGY-DISSIPATION Original Article LHCII complex PHOTOSYNTHETIC ANTENNA COMPLEX Life Sciences & Biomedicine HARVESTING-COMPLEX-II Plant Biology & Botany 0607 Plant Biology ANGSTROM RESOLUTION Fluorescence correlation spectroscopy Fluorescence spectroscopy 03 medical and health sciences PHOTOSYSTEM-II THYLAKOID MEMBRANES CHLOROPHYLL-A/B COMPLEX Science & Technology Quenching (fluorescence) Photoprotection Plant Sciences PROTEIN COMPLEX Photosystem II Protein Complex Cell Biology Fluorescence quenching Electrophoresis Crystallography Spectrometry Fluorescence 030104 developmental biology chemistry 010606 plant biology & botany |
| Zdroj: | Photosynthesis Research |
| ISSN: | 1573-5079 0166-8595 |
| Popis: | It emerges from numerous experiments that LHCII, the major photosynthetic antenna complex of plants, can appear not only in the trimeric or monomeric states but also as a dimer. We address the problem whether the dimeric form of the complex is just a simple intermediate element of the trimer–monomer transformation or if it can also be a physiologically relevant molecular organization form? Dimers of LHCII were analyzed with application of native electrophoresis, time-resolved fluorescence spectroscopy, and fluorescence correlation spectroscopy. The results reveal the appearance of two types of LHCII dimers: one formed by the dissociation of one monomer from the trimeric structure and the other formed by association of monomers into a distinctively different molecular organizational form, characterized by a high rate of chlorophyll excitation quenching. The hypothetical structure of such an energy quencher is proposed. The high light-induced LHCII dimerization is discussed as a potential element of the photoprotective response in plants. Electronic supplementary material The online version of this article (doi:10.1007/s11120-017-0387-6) contains supplementary material, which is available to authorized users. |
| Databáze: | OpenAIRE |
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