Replication of gene polymorphisms associated with periodontitis‐related traits in an elderly cohort: the Washington Heights/Inwood Community Aging Project Ancillary Study of Oral Health

Autor: Teresa Yang, Bin Cheng, James M. Noble, Christiane Reitz, Panos N. Papapanou
Rok vydání: 2022
Předmět:
Zdroj: J Clin Periodontol
ISSN: 1600-051X
0303-6979
DOI: 10.1111/jcpe.13605
Popis: AIMS: We sought to replicate findings from published genome-wide association studies, linking specific candidate gene loci with periodontitis-related clinical/microbial traits. METHODS: In the published GWAS, a total of 2,196 SNPs associated with periodontitis-related traits at a p≤5×10(−6) and mapped to 136 gene loci. The replication cohort included 1,124 individuals, 65–98 years old (67% female, 45% Hispanic, 30% Black, 23% White) with available genome-wide genotypes and full-mouth periodontal status. Microbial profiles using checkerboard DNA-DNA hybridization and 16SrRNA sequencing were available from 912 and 739 participants, respectively. FINDINGS: Using gene-specific p-values after linkage disequilibrium pruning, the following gene/phenotype associations replicated successfully: CLEC19A with edentulism and %teeth with PD≥4mm; IL37, HPVC1, TRPS1, ABHD12B, LDLRAD4, TGM3, GRK5 with %teeth with PD≥4mm; DAB2IP with presence of PD≥6mm; KIAA1715(LNPK), ROBO2, RAB28, LINC01017, NELL1, LDLRAD4(C18orF1), CRYBB2P1 with %teeth with CAL≥3 mm; RUNX2, LAMA2 with %teeth with CAL≥5 mm; and KIAA1715(LNPK) with high colonization by Aggregatibacter actinomycetemcomitans. In addition, CLEC19A, IQSEC1 and EMR1 associated with microbial abundance based on checkerboard data, LBP and NCR2 with abundance based on sequencing data, and NCR2 with microbial diversity based on sequencing data. CONCLUSIONS: Several gene loci identified in published GWAS as associated with periodontitis-related phenotypes replicated successfully in an elderly cohort.
Databáze: OpenAIRE
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