Identification of a novel gene signature of ES cells self-renewal fluctuation through system-wide analysis
| Autor: | Geppino Falco, Daniela Tagliaferri, Mario DeFelice, Luigi Cerulo, Filomena Russo, Pina Marotta, Pietro Zoppoli, Claudia Mazio, Michele Ceccarelli |
|---|---|
| Přispěvatelé: | Cerulo, L, Tagliaferri, D, Marotta, P, Zoppoli, P, Russo, F, Mazio, C, DE FELICE, Mario, Ceccarelli, M, Falco, Geppino |
| Jazyk: | angličtina |
| Rok vydání: | 2014 |
| Předmět: |
Cellular differentiation
Gene Expression lcsh:Medicine Ectoderm Signal transduction ERK signaling cascade Mice 0302 clinical medicine Molecular cell biology Genes Reporter Metastate lcsh:Science reproductive and urinary physiology Genetics 0303 health sciences Multidisciplinary Stem Cells Signaling cascades Cell Differentiation Cell biology medicine.anatomical_structure embryonic structures Endoderm Cellular Types Research Article Cell type Mesoderm Cell Potency Biology 03 medical and health sciences Artificial Intelligence medicine Animals Embryonic Stem Cells 030304 developmental biology Cell Proliferation urogenital system Gene Expression Profiling lcsh:R Computational Biology Embryonic stem cell Gene expression profiling Cell survival Pax8 Thyroid Transcription factor lcsh:Q Transcriptome 030217 neurology & neurosurgery Biomarkers Transcription Factors Developmental Biology |
| Zdroj: | PLoS ONE, Vol 9, Iss 1, p e83235 (2014) PLoS ONE PloS one 9 (2014). doi:10.1371/journal.pone.0083235 info:cnr-pdr/source/autori:Cerulo L.; Tagliaferri D.; Marotta P.; Zoppoli P.; Russo F.; Mazio C.; DeFelice M.; Ceccarelli M.; Falco G./titolo:Identification of a novel gene signature of ES cells self-renewal fluctuation through system-wide analysis/doi:10.1371%2Fjournal.pone.0083235/rivista:PloS one/anno:2014/pagina_da:/pagina_a:/intervallo_pagine:/volume:9 |
| ISSN: | 1932-6203 |
| Popis: | Embryonic Stem cells (ESCs) can be differentiated into ectoderm, endoderm, and mesoderm derivatives, producing the majority of cell types. In regular culture conditions, ESCs' self-renewal is maintained through molecules that inhibit spontaneous differentiation enabling long-term cellular expansion. This undifferentiating condition is characterized by multiple metastable states that fluctuate between self-renewal and differentiation balance. Here, we aim to characterize the high-pluripotent ESC metastate marked by the expression of Zscan4 through a supervised machine learning framework based on an ensemble of support vector machine (SVM) classifiers. Our study revealed a leukaemia inhibitor factor (Lif) dependent not-canonical pluripotency signature (AF067063, BC061212, Dub1, Eif1a, Gm12794, Gm13871, Gm4340, Gm4850, Tcstv1/3, and Zfp352), that specifically marks Zscan4 ESCs' fluctuation. This novel ESC metastate is enhanced by highpluripotency culture conditions obtained through Extracellular signal Regulated-Kinase (ERK) and Glycogen synthase kinase- 3 (Gsk-3) signaling inhibition (2i). Significantly, we reported that the conditional ablation of the novel ESC metastate marked by the expression of Gm12794 is required for ESCs self-renewal maintenance. In conclusion, we extend the comprehension of ESCs biology through the identification of a novel molecular signature associated to pluripotency programming. Copyright: © 2014 Cerulo et al. |
| Databáze: | OpenAIRE |
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