A Stress-Inducible Resveratrol O-Methyltransferase Involved in the Biosynthesis of Pterostilbene in Grapevine
| Autor: | Pere Mestre, Philippe Hugueney, Maria Santos-Rosa, Sabine Wiedemann-Merdinoglu, Anne Poutaraud, Emilce Prado, Didier Merdinoglu, Patricia Claudel, Francis Karst, Laure Schmidlin |
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| Přispěvatelé: | Santé de la vigne et qualité du vin (SVQV), Institut National de la Recherche Agronomique (INRA)-Université Louis Pasteur - Strasbourg I, École normale supérieure de Lyon (ENS de Lyon), École normale supérieure - Lyon (ENS Lyon) |
| Rok vydání: | 2008 |
| Předmět: |
MILDIOU
MALADIE FONGIQUE 0106 biological sciences Agroinfiltration Pterostilbene PLASMOPARA VITICOLA Physiology Nicotiana benthamiana Plant Science Biology Resveratrol 01 natural sciences [SDV.GEN.GPL]Life Sciences [q-bio]/Genetics/Plants genetics 03 medical and health sciences chemistry.chemical_compound GRAPEVINE Genetics Ultraviolet light BIOSYNTHESIS STILBENE MECANISME DE DEFENSE RESVERATROL 030304 developmental biology chemistry.chemical_classification O-METHYLTRANSFERASE 0303 health sciences PTEROSTILBENE Phytoalexin food and beverages biology.organism_classification EXPRESSION DES GENES O-methyltransferase PHYTOALEXINE Biochemistry chemistry Plasmopara viticola biology.protein 010606 plant biology & botany |
| Zdroj: | Plant Physiology Plant Physiology, 2008, 148 (3), pp.1630-1639. ⟨10.1104/pp.108.126003⟩ Plant Physiology, American Society of Plant Biologists, 2008, 148 (3), pp.1630-1639. ⟨10.1104/pp.108.126003⟩ |
| ISSN: | 1532-2548 0032-0889 |
| DOI: | 10.1104/pp.108.126003 |
| Popis: | International audience; Stilbenes are considered the most important phytoalexin group in grapevine (Vitis vinifera) and they are known to contribute to the protection against various pathogens. The main stilbenes in grapevine are resveratrol and its derivatives and, among these, pterostilbene has recently attracted much attention due both to its antifungal and pharmacological properties. Indeed, pterostilbene is 5 to 10 times more fungitoxic than resveratrol in vitro and recent studies have shown that pterostilbene exhibits anticancer, hypolipidemic, and antidiabetic properties. A candidate gene approach was used to identify a grapevine resveratrol O-methyltransferase (ROMT) cDNA and the activity of the corresponding protein was characterized after expression in Escherichia coli. Transient coexpression of ROMT and grapevine stilbene synthase in tobacco (Nicotiana benthamiana) using the agroinfiltration technique resulted in the accumulation of pterostilbene in tobacco tissues. Taken together, these results showed that ROMT was able to catalyze the biosynthesis of pterostilbene from resveratrol both in vitro and in planta. ROMT gene expression in grapevine leaves was induced by different stresses, including downy mildew (Plasmopara viticola) infection, ultraviolet light, and AlCl3 treatment. |
| Databáze: | OpenAIRE |
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