Microcalorimetric study of metabolic inhibition by humic acids in mitochondria from Oryctolagus cuniculus domestica liver cells
| Autor: | Chang li Xie, Song sheng Qu, Xiao qiang Wang, Feng jiao Deng, Yi Liu, Yu Guo |
|---|---|
| Rok vydání: | 1996 |
| Předmět: |
Isothermal microcalorimetry
Environmental Engineering Health Toxicology and Mutagenesis Mitochondria Liver Biology Mitochondrion Calorimetry chemistry.chemical_compound Environmental Chemistry Humic acid Animals Humic Substances chemistry.chemical_classification Lagomorpha Public Health Environmental and Occupational Health General Medicine General Chemistry Metabolism biology.organism_classification Pollution In vitro Biochemistry chemistry Liver Rabbits Thermogenesis Mathematics Toxicant Body Temperature Regulation |
| Zdroj: | Chemosphere. 33(1) |
| ISSN: | 0045-6535 |
| Popis: | The metabolic thermogenesis curves of mitochondria isolated from Oryctolagus cuniculus domestics liver tissue have been determined by using an LKB - 2277 bioactivity monitor. After isolation, mitochondria maintain activity when stored in appropriate nutrients. The thermokinetic equation: dP dt = k m · P 0 P t = P 0 + k m · t was estabolished for the activity recovery phase of metabolism. For these studies, the order of metabolism was found to be zero. The metabolic activity of mitochondria was inhibited by humic acid, a potential toxicant in Kashin - Beck and Keshan diseases. Experimental results indicate that humic acid will effect the heat output and lag time of mitochondrial metabolism. |
| Databáze: | OpenAIRE |
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