Improving the Diagnosis of Scrub Typhus by Combining groEL Based Polymerase Chain Reaction and IgM ELISA
| Autor: | Ashok C Naik, Karthikeyan Anitha Patricia, Nair Shashikala, S.L. Hoti, Reba Kanungo, Purushothaman Jambulingam |
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| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Orientia tsutsugamushi 030106 microbiology Clinical Biochemistry lcsh:Medicine Eschar Scrub typhus law.invention 03 medical and health sciences law medicine orientia tsutsugamushi Polymerase chain reaction laboratory diagnosis fever biology business.industry lcsh:R General Medicine medicine.disease biology.organism_classification bacterial infections and mycoses Virology Orientia GroEL Immunoglobulin M biology.protein medicine.symptom Antibody rickettsial infections business |
| Zdroj: | Journal of Clinical and Diagnostic Research, Vol 11, Iss 8, Pp DC27-DC31 (2017) |
| Popis: | Introduction: Scrub typhus, an acute febrile illness, caused by Orientia tsutsugamushi, is an important cause of pyrexia of unknown origin in regions of endemicity. This disease is mostly underdiagnosed or misdiagnosed, the reasons for this being a combination of factors which include clinical manifestations that can mimic other infections, lack of easy and reliable diagnostic methods and variation among strains in endemic areas. Hence, easy and reliable methods of diagnosis will contribute to rapid identification and treatment of the infection. Aim: The aim of the study was to compare four different methods of detection of scrub typhus and to identify one single test or a combination of tests detecting maximum number of cases. Materials and Methods: One hundred and forty-five suspected scrub typhus cases were included in this study. Duration of fever and presence of eschar in each patient was noted down. EnzymeLinked Immunosorbent Assay (ELISA) to detect Immunoglobulin M (IgM) antibodies and Polymerase Chain Reaction (PCR) to detect three genes of Orientia, namely, 56 kDa, 16S rRNA, and groEL were done on these samples. The results of each test were analyzed to identify the test picking up maximum number of positive samples. Statistical analysis was performed using Chi-square test. The level of significance was set at p |
| Databáze: | OpenAIRE |
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