Nucleotide variation in Sabin type 2 poliovirus from an immunodeficient patient with poliomyelitis

Autor: Valentina Donati, Javier Martin, Stefano Fiore, R. Vivarelli, Paolo Balestri, Francis Delpeyroux, Alessandro Plebani, Gabriele Buttinelli, Anna Rosa Soresina, Jill Marturano, Lucia Fiore
Přispěvatelé: Laboratory of Virology, Istituto Superiore di Sanita [Rome], Department of Pediatrics, University of Brescia, Pediatrics Unit, University of Sienna, Epidémiologie Moléculaire des Entérovirus, Institut Pasteur [Paris], National Institute for Biological Standards and Control (NIBSC), Medicines and Healthcare Products Regulatory Agency (MHRA), Istituto Superiore di Sanità (ISS), Institut Pasteur [Paris] (IP)
Rok vydání: 2003
Předmět:
MESH: Sequence Analysis
DNA
viruses
MESH: Virulence
medicine.disease_cause
MESH: Variation (Genetics)
Feces
Mice
chemistry.chemical_compound
Agammaglobulinemia
Paralysis
MESH: Animals
MESH: Capsid Proteins
0303 health sciences
Virulence
Poliovirus
MESH: Feces
virus diseases
MESH: Amino Acid Substitution
3. Good health
Poliomyelitis
Child
Preschool
[SDV.MP.VIR]Life Sciences [q-bio]/Microbiology and Parasitology/Virology
MESH: 5' Untranslated Regions
medicine.symptom
MESH: Poliovirus
medicine.drug_class
Molecular Sequence Data
Reversion
Biology
Vaccines
Attenuated
complex mixtures
Virus
Microbiology
03 medical and health sciences
Virology
MESH: Vaccines
Attenuated
medicine
Animals
Humans
MESH: Agammaglobulinemia
Viral shedding
MESH: Mice
030304 developmental biology
MESH: Humans
MESH: Molecular Sequence Data
030306 microbiology
MESH: Child
Preschool
Genetic Variation
Pleconaril
Sequence Analysis
DNA
medicine.disease
Amino Acid Substitution
MESH: Poliovirus Vaccine
Oral
chemistry
Poliovirus Vaccine
Oral
MESH: Poliomyelitis
Capsid Proteins
Antiviral drug
5' Untranslated Regions
Zdroj: Journal of General Virology
Journal of General Virology, Microbiology Society, 2003, 84 (Pt 5), pp.1215-21. ⟨10.1099/vir.0.18974-0⟩
Journal of General Virology, 2003, 84 (Pt 5), pp.1215-21. ⟨10.1099/vir.0.18974-0⟩
ISSN: 1465-2099
0022-1317
DOI: 10.1099/vir.0.18974-0
Popis: The molecular and antigenic properties of a Sabin-like type 2 poliovirus, isolated from the stool samples of a 2-year-old agammaglobulinaemic child who developed paralysis 1 year after receiving the third dose of oral poliovirus vaccine, were analysed. The virus revealed 0.88 % genome variation in the VP1 region compared with the standard reference strain, compatible with replication of the virus in the intestine over approximately 1 year. The typical mutations in the 5'NCR and VP1 associated with reversion to neurovirulence for Sabin type 2 poliovirus were found. Despite this, the virus was characterized by both PCR and ELISA tests as Sabin-like and showed temperature sensitivity and neurovirulence in transgenic mice typical of the Sabin type 2 vaccine strain. Gammaglobulin replacement therapy led rapidly to virus clearance, which, when combined with treatment with the antiviral drug pleconaril, stopped virus excretion; no further virus shedding occurred. This is the first case of poliomyelitis and long-term excretion from an immunodeficient patient to be reported in Italy through the active 'Acute Flaccid Paralysis' surveillance system.
Databáze: OpenAIRE
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