Učinak mješavine biljnih ekstrakata i metotreksata na supresiju adjuvantnog artritisa u štakora

Autor: Rūta Bradūnaitė, Laima Leonavičienė, Irena Dumalakienė, Rita Vilienė, Irena Jonauskienė, Laimis Akramas, Miglė Leonavičiūtė Klimantavičienė, Zygmunt Mackiewicz, Audrius Vasiliauskas
Jazyk: angličtina
Rok vydání: 2021
Předmět:
Zdroj: Veterinarski arhiv
Volume 91
Issue 4
ISSN: 1331-8055
0372-5480
Popis: The present study evaluated the therapeutic benefits of complex herbal preparation named CBMDS, consisting of turmeric (Curcuma longa), Boswellia (Boswellia serrata), Methylsulphonylmethane, Devil’s Claw (Harpagophytum procumbens) and Silymarin, using it in combination with methotrexate, in order to suppress adjuvant arthritis in rats, and to attenuate methotrexate-induced liver damage. Adjuvant arthritis was induced in 28 rats by a single subplantar injection of complete Freund’s adjuvant (0.1 mL) into the left hind paw. The animals were divided into four groups (with seven animals in each). Group I received CBMDS, Group II - CBMDS in combination with methotrexate, and Group III just methotrexate. The treatment lasted from day 0 to day 17 (CBMDS was given daily except weekends in a dose of 160 mg/kg, methotrexate - 2 mg/kg once a week). Group IV was the control group. Clinical (body weight, hind paw volume, erythrocyte sedimentation rate, leukocyte count), biochemical (serum pro-/antioxidant activity markers), immunological (serum interleukin levels) and histological changes in joint and liver tissues were evaluated. CBMDS significantly alleviated arthritis and reduced hepatic damage, which was more evident in the methotrexate group. The combined treatment also markedly reduced arthritic symptoms and levels of malondialdehyde. Antioxidant activity was significantly higher in treated Groups I and II. CBMDS and its combination with methotrexate promoted anti- arthritic action, reduced histological changes in the joint tissues, and minimized methotrexate-induced liver toxicity.
U ovom je radu procijenjena terapijska korist mješavine biljnih preparata pod imenom CBMDS, koja sadržava kurkumu (Curcuma longa), tamjanovo drvo (Boswellia serrata), metilsulfonilmetan, vražju kandžu (Harpagophytum procumbens) i silimarin. Biljni preparati su upotrijebljeni u kombinaciji s metotrekstom s ciljem supresije adjuvantnog artritisa u štakora i smanjenja oštećenja jetre uzrokovanog metotreksatom. Adjuvantni artritis izazvan je u 28 štakora pojedinačnom subplantarnom injekcijom mineralne uljne emulzije Freund’s Complete Adjuvant (0,1 mL) u stražnju lijevu šapu. Životinje su podijeljene u četiri skupine (u svakoj po sedam jedinki). Skupina I primila je CBMDS, skupina II – CBMDS u kombinaciji s metotreksatom, a skupina III – metotreksat. Pokus je trajao od nultog do 17. dana (CBMDS je davan svaki dan, osim vikendom, u dozi od 160 mg/kg, metotreksat u dozi od 2 mg/kg jedanput tjedno). Skupina IV bila je kontrolna skupina. Procijenjene su kliničke (tjelesna masa, opseg stražnje šape, sedimetacija eritrocita, broj leukocita), biokemijske (marker serumske aktivnosti prooksidansa i antioksidansa), imunološke (razine serumskog interleukina) i histološke promjene u zglobovima i jetri. CBMDS je znakovito ublažio artritis i smanjio oštećenje jetre, što je bilo očitije u skupini s metotreksatom. Kombinirana terapija također je znatno smanjila simptome artritisa i razine malondialdehida. Antioksidacijska aktivnost bila je znakovito veća u skupinama I i II. CBMDS u kombinaciji s metotreksatom imao je antiartritično djelovanje, smanjio je histološke promjene u zglobovima i minimizirao toksičnost metotreksata za jetru.
Databáze: OpenAIRE
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