A physical and genetic map of Cannabis sativa identifies extensive rearrangements at the THC/CBD acid synthase loci
| Autor: | Larry Holbrook, Timothy P. Hughes, Gintaras Deikus, Robert Sebra, Kaitlin U. Laverty, Jake Stout, Hardik Shah, Jonathan E. Page, Mitchell J. Sullivan, Harm van Bakel, Navdeep Gill |
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| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
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medicine.medical_treatment Prenyltransferase Chromosomes Plant Ligases 03 medical and health sciences 0302 clinical medicine Genetics medicine Allele Repeated sequence Gene Genetics (clinical) 030304 developmental biology Cannabis Plant Proteins 2. Zero hunger Gene Rearrangement 0303 health sciences biology Cannabinoids Chromosome Chromosome Mapping Gene rearrangement biology.organism_classification Cannabinoid 030217 neurology & neurosurgery |
| Popis: | Cannabis sativa is widely cultivated for medicinal, food, industrial, and recreational use, but much remains unknown regarding its genetics, including the molecular determinants of cannabinoid content. Here, we describe a combined physical and genetic map derived from a cross between the drug-type strain Purple Kush and the hemp variety “Finola.” The map reveals that cannabinoid biosynthesis genes are generally unlinked but that aromatic prenyltransferase (AP), which produces the substrate for THCA and CBDA synthases (THCAS and CBDAS), is tightly linked to a known marker for total cannabinoid content. We further identify the gene encoding CBCA synthase (CBCAS) and characterize its catalytic activity, providing insight into how cannabinoid diversity arises in cannabis. THCAS and CBDAS (which determine the drug vs. hemp chemotype) are contained within large (>250 kb) retrotransposon-rich regions that are highly nonhomologous between drug- and hemp-type alleles and are furthermore embedded within ∼40 Mb of minimally recombining repetitive DNA. The chromosome structures are similar to those in grains such as wheat, with recombination focused in gene-rich, repeat-depleted regions near chromosome ends. The physical and genetic map should facilitate further dissection of genetic and molecular mechanisms in this commercially and medically important plant. |
| Databáze: | OpenAIRE |
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