In vitro Evaluation of Buffy-Coat-Derived Platelet Concentrates Stored in a Synthetic Medium
| Autor: | R. Fijnheer, C. W. N. Gouwerok, Christa H. E. Homburg, Dirk Roos, M. N. Boomgaard, H. A. Veldman, Dirk de Korte, A.J.M. van den Eertwegh |
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| Přispěvatelé: | Medical oncology |
| Rok vydání: | 1991 |
| Předmět: |
Blood Platelets
Platelet Aggregation Cell Survival medicine.drug_class Intracellular pH Centrifugation Biological activity Cell Separation Buffy coat Hematology General Medicine Hydrogen-Ion Concentration Monoclonal antibody In vitro Plasma chemistry.chemical_compound Adenosine Triphosphate Biochemistry chemistry Blood Preservation medicine Humans Platelet Quantitative analysis (chemistry) Adenosine triphosphate |
| Zdroj: | Fijnheer, R, Veldman, H A, van den Eertwegh, A J M, Gouwerok, C W N, Homburg, C H E, Boomgaard, M N, de Korte, D & Roos, D 1991, ' In vitro Evaluation of Buffy-Coat-Derived Platelet Concentrates Stored in a Synthetic Medium ', Vox Sanguinis, vol. 60, no. 1, pp. 16-22 . https://doi.org/10.1111/j.1423-0410.1991.tb00865.x Vox Sanguinis, 60(1), 16-22. Wiley-Blackwell |
| ISSN: | 1423-0410 0042-9007 |
| DOI: | 10.1159/000461241 |
| Popis: | Human blood platelets, prepared by the buffy‐coat method, were prepared and stored in different synthetic media. In a synthetic medium based on gluconate, acetate and citrate (GAC), the pH was 6.8 on day 6. This medium was chosen for further evaluation. The total platelet count and the leukocyte contamination were significantly lower in the platelet concentrates (PCs) prepared in GAC compared to PCs prepared in plasma. Platelets stored in plasma or in GAC were equally functional when tested for aggregation and adenosine triphosphate (ATP) secretion. Only stimuli that act through the arachidonic‐acid pathway induced a lower platelet response in GAC. Platelet morphology was quantified by measuring the difference in light transmission during stirring at different rates in an aggregometer; no significant differences for platelets stored in GAC as compared to plasma were observed. Activation of platelets was measured by binding of monoclonal antibodies (McAb) against the Gp IIb/IIIa complex and against activation‐dependent antigens (GMP 140 from the α‐granules and a 53‐kD glycoprotein from the lysosomal granules). There was no difference in binding of these McAb between platelets prepared and stored in plasma or GAC. We conclude that platelets prepared by the buffy‐coat method and stored in GAC have the same in vitro qualitities as platelets stored in plasma, except for the lower aggregation response by the archidonic‐acid pathway. This is probably due to an acetate‐induced decrease in intracellular pH. |
| Databáze: | OpenAIRE |
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