Activated Ki-ras proto-oncogene in spontaneously transformed and chemical tumor-derived cell lines related to the mouse lung alveologenic carcinoma
| Autor: | Garry J. Smith, Jacqueline M. Bentel, Juliet O. Miller, Vicki Ferguson, Don A. Leigh |
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| Rok vydání: | 1990 |
| Předmět: |
Adenoma
Cancer Research Lung Neoplasms Molecular Sequence Data Oligonucleotides Biology medicine.disease_cause Polymerase Chain Reaction Urethane law.invention Proto-Oncogene Proteins p21(ras) Mice law Complementary DNA Tumor Cells Cultured Carcinoma medicine Animals RNA Messenger RNA Neoplasm Neoplasm Metastasis Codon Molecular Biology Polymerase chain reaction Mutation Base Sequence Oncogene Transition (genetics) Point mutation Oncogenes Adenocarcinoma Bronchiolo-Alveolar medicine.disease Molecular biology Cell Transformation Neoplastic Cell culture Neoplasm Transplantation |
| Zdroj: | Molecular Carcinogenesis. 3:387-392 |
| ISSN: | 1098-2744 0899-1987 |
| DOI: | 10.1002/mc.2940030611 |
| Popis: | An in vitro cell model of mouse lung alveologenic carcinoma consisting of preneoplastic nonmalignant cells, spontaneously transformed cells, and urethane-induced malignant cells was analyzed for phenotypic and genotypic changes associated with the transition to neoplasia. The polymerase chain reaction (PCR) was used to amplify the cDNA derived from the c-Ki-ras mRNA corresponding to exons 1 and 2 of the proto-oncogene. This approach allowed analysis of the gene transcription product rather than potentially unexpressed DNA. Direct sequencing of the PCR product identified a common single point mutation, alone or together with wild-type mRNA for c-Ki-ras, in all of the malignant clones. An A----G transition in the second base position of codon 61 was common to spontaneously malignant and chemical tumor-derived cell lines and to lines selected for lung metastatic behavior. The absence of the mutation in the nonmalignant cells suggests that the Ki-ras mutation may be a factor in onset or maintenance of the malignant phenotype and, at least in vitro, may be a late event in the transformation process. |
| Databáze: | OpenAIRE |
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