Effects of Pirfenidone and Collagen-Polyvinylpyrrolidone on Macroscopic and Microscopic Changes, TGF-β1 Expression, and Collagen Deposition in an Experimental Model of Tracheal Wound Healing
| Autor: | Ivette Buendía-Roldan, J. Raúl Olmos-Zúñiga, Laura Romero-Romero, Jazmin Jasso-Arenas, Jazmin Calyeca-Gómez, Axel E. Guzmán-Cedillo, Matilde Baltazares-Lipp, Claudia Hernández-Jiménez, Mariana Silva-Martínez, Rogelio Jasso-Victoria, Miguel Gaxiola-Gaxiola, Alan de Jesús Martínez-Salas |
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| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Pathology medicine.medical_specialty Article Subject medicine.medical_treatment Scars lcsh:Medicine Inflammation General Biochemistry Genetics and Molecular Biology 03 medical and health sciences 0302 clinical medicine Fibrosis medicine Saline General Immunology and Microbiology business.industry Mitomycin C lcsh:R General Medicine Pirfenidone medicine.disease Tracheal Stenosis 030104 developmental biology 030220 oncology & carcinogenesis medicine.symptom business Wound healing medicine.drug |
| Zdroj: | BioMed Research International, Vol 2017 (2017) |
| ISSN: | 2314-6141 2314-6133 |
| Popis: | Tracheal stenosis (TS) is a fibrosis originated by prolonged inflammation and increased transforming growth factor beta 1 (TGF-β1) expression and collagen deposition (CD) in the tracheal wound. Several wound-healing modulators (WHMs) have been used to modulate the tracheal healing process and prevent TS, but they have failed, justifying the need to evaluate alternative WHM. The pirfenidone (PFD) and collagen-polyvinylpyrrolidone (Collagen-PVP) decrease inflammation and fibrosis. This study assessed the effect of PFD administration and Collagen-PVP topical application on macroscopic and microscopic changes, TGF-β1 expression, and CD in an experimental model of tracheal wound healing. Forty Wistar rats underwent cervical tracheoplasty, were divided into 4 groups (n=10), and were treated with different WHM: group I, saline solution (SS); group II, Collagen-PVP; group III, mitomycin C (MMC); and group IV, 40 mg/kg PFD. Four weeks after surgery, the macroscopic and microscopic changes, in situ TGF-β1 expression, and CD in posttracheoplasty scars were evaluated. The animals treated with Collagen-PVP and PFD developed less inflammation and fibrosis than animals in the other study groups (p<0.05, Kruskal-Wallis) and, moreover, showed lower TGF-β1 expression and CD than animals in group I (p<0.05, ANOVA and Tukey’s test). In conclusion, PFD and Collagen-PVP decrease inflammation, fibrosis, TGFβ-1 expression, and CD in the posttracheoplasty rats’ scar. |
| Databáze: | OpenAIRE |
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