Cholangiocyte marker-positive and -negative fetal liver cells differ significantly in their ability to regenerate the livers of adult rats exposed to retrorsine
| Autor: | Kate E. Brilliant, Helen M. Callanan, Edmond Sabo, Rhonda Simper-Ronan, Douglas C. Hixson, Donna L. Flanagan, Marie P. Carreiro |
|---|---|
| Rok vydání: | 2006 |
| Předmět: |
medicine.drug_class
Cell Transplantation Dipeptidyl Peptidase 4 Cell Spleen Biology Immunofluorescence Monoclonal antibody Cholangiocyte Epitope medicine Animals Cell Lineage Molecular Biology Pyrrolizidine Alkaloids Fetus medicine.diagnostic_test Immunomagnetic Separation Antibodies Monoclonal Cell Differentiation Epithelial Cells Molecular biology Antineoplastic Agents Phytogenic Rats Inbred F344 Liver Regeneration Rats carbohydrates (lipids) medicine.anatomical_structure Phenotype Liver Hepatocyte Immunology Hepatocytes Biomarkers Developmental Biology |
| Zdroj: | Development (Cambridge, England). 133(21) |
| ISSN: | 0950-1991 |
| Popis: | We have used monoclonal antibodies against cell-surface developmental epitopes in combination with micromagnetic beads to isolate phenotypically defined subpopulations of cholangiocyte marker-positive fetal liver epithelial cells (CMP-FLEC). Differentiation potential was evaluated by injecting cell isolates from dipeptidyl peptidase IV (DPPIV) positive (DPPIV+) Fischer donor rats into the spleen of partially hepatectomized, DPPIV negative (DPPIV-)Fischer host rats exposed to retrorsine. At various time points, liver tissue was harvested and cells in DPPIV+ colonies were phenotyped by immunofluorescence and histochemical protocols. Functional differentiation and liver replacement were determined by comparing donor and host hepatocyte protein expression patterns and DPPIV enzyme activity in extracts from livers of host rats receiving CMP-FLEC. Our results showed that bipotentiality was retained during differentiation and maturation of CMP-FLEC, indicating that the acquisition of ductal morphology and phenotype were not indicative of lineage commitment. CMP-FLEC transplanted into the adult rat liver lost ductal and gained hepatocyte markers, and acquired protein expression patterns in 2D gels with a close similarity (>75% spot match) to host hepatocytes but differing significantly from the transplanted CMP-FLEC cell isolate ( |
| Databáze: | OpenAIRE |
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