Illuminating glycoscience: synthetic strategies for FRET-enabled carbohydrate active enzyme probes
| Autor: | Eoin M. Scanlan, Gavin J. Miller, Meenakshi Singh, Michael Watkinson |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
chemistry.chemical_classification
CAZy 010405 organic chemistry Chemistry Biomolecule Computational biology Carbohydrate 010402 general chemistry 01 natural sciences Biochemistry Genetics and Molecular Biology (miscellaneous) Biochemistry 0104 chemical sciences chemistry.chemical_compound Förster resonance energy transfer Chemistry (miscellaneous) QD Glycosyl Molecular Biology Active enzyme Carbohydrate active enzymes |
| Zdroj: | RSC Chemical Biology. 1:352-368 |
| ISSN: | 2633-0679 |
| Popis: | Carbohydrates are an essential class of biomolecule and carbohydrate active enzymes (CAZys) catalyse their synthesis, refinement, and degradation, hence contributing an overall regulatory capacity to their underpinning physiological roles. As such, there is a considerable current requirement to be able to monitor, quantify and inhibit CAZy activity. It is here that Forster resonance energy transfer (FRET) is emerging as a powerful tool in enabling this, through synthetic conjugation of appropriate fluorogenic partners into a particular CAZy substrate. In this review we survey recent accomplishments in synthetic methodology for accessing defined carbohydrate structures, suitably equipped with FRET probe capability, followed by their utilisation in studying particular classes of CAZy. The majority of examples concern endo-acting glycosidases, but emergent probes for exo-glycosidases, glycosyl transferases and catalytic antibodies are also examined. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|