Changes in protein expression profiles in bovine endometrial epithelial cells exposed to E coli LPS challenge
| Autor: | Gilles Charpigny, M. Chanrot, Luigi Bonizzi, Alessio Soggiu, Patrice Humblot, Cristian Piras, Andrea Urbani, Viviana Greco, Paola Roncada, Yongzhi Guo |
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| Přispěvatelé: | Dipartimento di Medicina Veterinaria, Università degli studi di Milano [Milano], Division of Reproduction, Department of Clinical Sciences, Swedish University of Agricultural Sciences (SLU), Rajamangala University of Technology, Proteomics and Metabonomics Unit, IRCCS Santa Lucia Foundation, Istituto di Biochimica e Biochimica Clinica, Università Cattolica del Sacro Cuore, Biologie du développement et reproduction (BDR), École nationale vétérinaire d'Alfort (ENVA)-Institut National de la Recherche Agronomique (INRA)-Centre National de la Recherche Scientifique (CNRS), Istituto Sperimentale Italiano Lazzaro Spallanzani, Rajamangala University of Te chnology Srivijaya (RMUTSV, Thailand), European Project: 311776, Dipartimento di Medicina Veterinaria (DIMEVET), Università degli Studi di Milano [Milano] (UNIMI), Università cattolica del Sacro Cuore [Milano] (Unicatt), Biologie du Développement et Reproduction (BDR), École nationale vétérinaire d'Alfort (ENVA)-Institut National de la Recherche Agronomique (INRA), Istituto Spallanzani, Università degli Studi di Milano = University of Milan (UNIMI), Rajamangala University of Technology Thanyaburi (RMUTT), École nationale vétérinaire - Alfort (ENVA)-Institut National de la Recherche Agronomique (INRA) |
| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
Lipopolysaccharides
Proteomics 0301 basic medicine mammary gland bovin Proteome Lipopolysaccharide Workflow chemistry.chemical_compound Protein Interaction Mapping Gene expression Protein Interaction Maps endometrium [SDV.BDD]Life Sciences [q-bio]/Development Biology 2. Zero hunger uterine disease Biologie du développement cellule épithéliale Development Biology 3. Good health endomètre Female medicine.symptom escherichia coli Bacterial outer membrane Metabolic Networks and Pathways Biotechnology gene expression ovarian function Cell Survival Inflammation Biology 03 medical and health sciences medicine Matrix-Assisted Laser Desorption-Ionization Animals Settore BIO/10 - BIOCHIMICA Molecular Biology Spectrometry Cell growth epithelial cell Epithelial Cells Mass Molecular biology In vitro 030104 developmental biology chemistry Apoptosis Spectrometry Mass Matrix-Assisted Laser Desorption-Ionization Immunology Cattle Energy Metabolism |
| Zdroj: | Molecular BioSystems Molecular BioSystems, 2017, 13 (2), pp.392-405. ⟨10.1039/c6mb00723f⟩ Molecular BioSystems 2 (13), 392-405. (2017) |
| ISSN: | 1742-2051 |
| DOI: | 10.1039/c6mb00723f⟩ |
| Popis: | E. coli is one of the most frequently involved bacteria in uterine diseases. Lipopolysaccharide (LPS) is a component of the outer membrane of Gram-negative bacteria involved in pathogenic processes leading to post-partum metritis and endometritis in cattle. It also causes inflammation of the endometrium. The increase of cell proliferation by LPS is part of the inflammatory process. The aim of this study was to investigate possible changes in protein expression in relation to the proliferative response of bEECs after challenge with E. coli-LPS. In vitro culture of bEECs was performed from cow genital tracts collected at a slaughterhouse. In passage 5, bEECs from each of 9 cows (3 series of 3 cows) were exposed to 0, 8, and 16 μg ml(-1) LPS for 72 h. At time 0 and 72 h later, attached cells/living cells were counted and for each time and LPS dosage, cells were frozen for proteomic analyses. All samples from the 3 series were analyzed by 2-D gel electrophoresis coupled to MALDI-TOF/TOF mass spectrometry. The samples from the first series were subjected to shotgun nLC-MS/MS analysis. From the whole differential proteomics analysis, 38 proteins were differentially expressed (p < 0.05 to p < 0.001) following exposure to LPS. Among them, twenty-eight were found to be up-regulated in the LPS groups in comparison to control groups and ten were down-regulated. Differentially expressed proteins were associated with cell proliferation and apoptosis, transcription, destabilization of cell structure, oxidative stress, regulation of histones, allergy and general cell metabolism pathways. The de-regulations induced by LPS were consistent with the proliferative phenotype and indicated strong alterations of several cell functions. In addition, some of the differentially expressed proteins relates to pathways activated at the time of implantation. The specific changes induced through those signals may have negative consequences for the establishment of pregnancy. |
| Databáze: | OpenAIRE |
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