Isolation and characterization of a moderately halophilic Marinobacter phage-host system from the Arabian Sea
| Autor: | Ammini Parvathi, Arya Kaniyassery, Sreekumar Aparna |
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| Rok vydání: | 2019 |
| Předmět: |
DNA
Bacterial 010504 meteorology & atmospheric sciences 010501 environmental sciences Management Monitoring Policy and Law medicine.disease_cause 01 natural sciences Enrichment culture Microbiology RNA Ribosomal 16S Gammaproteobacteria Marinobacter medicine Bacteriophages Marinobacter salsuginis Phylogeny 0105 earth and related environmental sciences General Environmental Science Base Composition biology Strain (chemistry) Chemistry Fatty Acids General Medicine Sequence Analysis DNA 16S ribosomal RNA biology.organism_classification Pollution Halophile Salinity Environmental Monitoring |
| Zdroj: | Environmental monitoring and assessment. 192(3) |
| ISSN: | 1573-2959 |
| Popis: | Marinobacter is an ecologically important genus of Gammaproteobacteria found in diverse marine habitats, many species of which are capable of degrading hydrocarbons. In this study, we isolated a Marinobacter phage-host system from the surface waters of the Arabian Sea using enrichment culture methods, studied their growth characteristics and investigated the effect of salinity and nitrate concentrations on phage-host interactions. The bacterial isolate had maximum identity to Marinobacter salsuginis based on 16S rRNA similarities and was termed as Marinobacter sp., strain D1S9. It could tolerate up to 14% of NaCl with maximum growth at 11% NaCl. The host grew optimally between 35 and 40 °C and at pH 8. It had a generation time of 3.7 h with a mean growth rate of 0.27 h−1. The phage infected the host forming clear, round plaques of 1–2 mm diameter. It had a narrow host range restricted to the strain Marinobacter D1S9. The latent period and burst size of the phage were estimated to be 30 min and 106 phages per infected cell, respectively. The phage had an adsorption rate of 3.4 × 10−8 ml min−1 and retained 40.4% of its adsorption efficiency at 16% NaCl with a maximum at 4% NaCl (76.1%). Inorganic nitrate was found to have a direct role in controlling host growth and phage burst size. |
| Databáze: | OpenAIRE |
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