Ivermectin reduces in vivo coronavirus infection in a mouse experimental model
| Autor: | Romina Pagotto, Jorge Luis Pórfido, Hellen Daghero, Marcelo Hill, B. Varela, Ana Paula Arévalo, M. Duhalde Vega, Martina Crispo, Mercedes Segovia, Mariela Bollati-Fogolín, Kanji Yamasaki, José Manuel Verdes |
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| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Neutrophils viruses Diseases medicine.disease_cause Kidney Gastroenterology Monocytes Ivermectin Signs and symptoms Coronavirus Sars-Cov-2 Mice Inbred BALB C Multidisciplinary biology purl.org/becyt/ford/3.1 [https] Viral Load medicine.anatomical_structure Liver Medicine purl.org/becyt/ford/3 [https] Tumor necrosis factor alpha Female Coronavirus Infections Viral load medicine.drug medicine.medical_specialty Science 030106 microbiology Antiviral Agents Article ivermectin 03 medical and health sciences Mouse hepatitis virus In vivo Internal medicine medicine Animals Transaminases Murine hepatitis virus business.industry Tumor Necrosis Factor-alpha Body Weight COVID-19 Kidney metabolism Proteins biology.organism_classification Disease Models Animal 030104 developmental biology business |
| Zdroj: | Scientific Reports Scientific Reports, Vol 11, Iss 1, Pp 1-12 (2021) CONICET Digital (CONICET) Consejo Nacional de Investigaciones Científicas y Técnicas instacron:CONICET |
| ISSN: | 2045-2322 |
| Popis: | SARS-CoV2 is a single strand RNA virus member of the type 2 coronavirus family, responsible for causing COVID-19 disease in humans. The objective of this study was to test the ivermectin drug in a murine model of coronavirus infection using a type 2 family RNA coronavirus similar to SARS-CoV2, the mouse hepatitis virus (MHV). BALB/cJ female mice were infected with 6,000 PFU of MHV-A59 (Group Infected; n=20) and immediately treated with one single dose of 500 μg/kg of ivermectin (Group Infected + IVM; n=20), or were not infected and treated with PBS (Control group; n=16). Five days after infection/treatment, mice were euthanized to obtain different tissues to check general health status and infection levels. Overall results demonstrated that viral infection induces the typical MHV disease in infected animals, with livers showing severe hepatocellular necrosis surrounded by a severe lymphoplasmacytic inflammatory infiltration associated with a high hepatic viral load (52,158 AU), while ivermectin administration showed a better health status with lower viral load (23,192 AU; p |
| Databáze: | OpenAIRE |
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