Novel type of interstitial cell (Cajal-like) in human fallopian tube
Autor: | R. I. Braga, Mihaela Gherghiceanu, Leon Zagrean, N. Ionescu, Dragos Cretoiu, Eugen Radu, Mihai Ceausu, Carmen Ardeleanu, Sanda M. Ciontea, L. M. Popescu, Florina Vasilescu, Mihail Eugen Hinescu |
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Rok vydání: | 2005 |
Předmět: |
Cytoplasm
Pathology Antigens CD34 Cell Count Vimentin Basement Membrane CD57 Antigens Nerve Fibers Intermediate Filament Proteins Caveolae Cells Cultured Connective Tissue Cells education.field_of_study Histocytochemistry S100 Proteins Mitochondria Electrophysiology Proto-Oncogene Proteins c-kit Intercellular Junctions medicine.anatomical_structure symbols Molecular Medicine Female Ubiquitin Thiolesterase medicine.medical_specialty Population Biology Caveolins Article Interstitial cell symbols.namesake Microscopy Electron Transmission Telocyte Chromogranins medicine Humans education Fallopian Tubes Cell Nucleus Lamina propria Mucous Membrane Staining and Labeling Muscle Smooth Cell Biology Actins Interstitial cell of Cajal Microscopy Fluorescence Ultrastructure biology.protein Blood Vessels Chromogranin A Cell Surface Extensions |
Zdroj: | Journal of Cellular and Molecular Medicine. 9:479-523 |
ISSN: | 1582-4934 1582-1838 |
DOI: | 10.1111/j.1582-4934.2005.tb00376.x |
Popis: | We describe here--presumably for the first time--a Cajal-like type of tubal interstitial cells (t-ICC), resembling the archetypal enteric ICC. t-ICC were demonstrated in situ and in vitro on fresh preparations (tissue cryosections and primary cell cultures) using methylene-blue, crystal-violet, Janus-Green B or MitoTracker-Green FM Probe vital stainings. Also, t-ICC were identified in fixed specimens by light microscopy (methylene-blue, Giemsa, trichrome stainings, Gomori silver-impregnation) or transmission electron microscopy (TEM). The positive diagnosis of t-ICC was strengthened by immunohistochemistry (IHC; CD117/c-kit+ and other 14 antigens) and immunofluorescence (IF; CD117/c-kit+ and other 7 antigens). The spatial density of t-ICC (ampullar-segment cryosections) was 100-150 cells/mm2. Non-conventional light microscopy (NCLM) of Epon semithin-sections revealed a network-like distribution of t-ICC in lamina propria and smooth muscle meshwork. t-ICC appeared located beneath of epithelium, in a 10-15 microm thick 'belt', where 18+/-2% of cells were t-ICC. In the whole lamina propria, t-ICC were about 9%, and in muscularis approximately 7%. In toto, t-ICC represent ~8% of subepithelial cells, as counted by NCLM. In vitro, t-ICC were 9.9+/-0.9% of total cell population. TEM showed that the diagnostic 'gold standard' (Huizinga et al., 1997) is fulfilled by 'our' t-ICC. However, we suggest a 'platinum standard', adding a new defining criterion- characteristic cytoplasmic processes (number: 1-5; length: tens of microm; thickness: < or =0.5 microm; aspect: moniliform; branching: dichotomous; organization: network, labyrinthic-system). Quantitatively, the ultrastructural architecture of t-ICC is: nucleus, 23.6+/-3.2% of cell volume, with heterochromatin 49.1+/-3.8%; mitochondria, 4.8+/-1.7%; rough and smooth endoplasmic-reticulum (1.1+/-0.6%, 1.0+/-0.2%, respectively); caveolae, 3.4+/-0.5%. We found more caveolae on the surface of cell processes versus cell body, as confirmed by IF for caveolins. Occasionally, the so-called 'Ca2+-release units' (subplasmalemmal close associations of caveolae+endoplasmic reticulum+mitochondria) were detected in the dilations of cell processes. Electrophysiological single unit recordings of t-ICC in primary cultures indicated sustained spontaneous electrical activity (amplitude of membrane potentials: 57.26+/-6.56 mV). Besides the CD117/c-kit marker, t-ICC expressed variously CD34, caveolins 1&2, alpha-SMA, S-100, vimentin, nestin, desmin, NK-1. t-ICC were negative for: CD68, CD1a, CD62P, NSE, GFAP, chromogranin-A, PGP9.5, but IHC showed the possible existence of (neuro)endocrine cells in tubal interstitium. We call them 'JF cells'. In conclusion, the identification of t-ICC might open the door for understanding some tubal functions, e.g. pace-making/peristaltism, secretion (auto-, juxta- and/or paracrine), regulation of neurotransmission (nitrergic/purinergic) and intercellular signaling, via the very long processes. Furthermore, t-ICC might even be uncommitted bipotential progenitor cells. |
Databáze: | OpenAIRE |
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