Novel type of interstitial cell (Cajal-like) in human fallopian tube

Autor: R. I. Braga, Mihaela Gherghiceanu, Leon Zagrean, N. Ionescu, Dragos Cretoiu, Eugen Radu, Mihai Ceausu, Carmen Ardeleanu, Sanda M. Ciontea, L. M. Popescu, Florina Vasilescu, Mihail Eugen Hinescu
Rok vydání: 2005
Předmět:
Cytoplasm
Pathology
Antigens
CD34

Cell Count
Vimentin
Basement Membrane
CD57 Antigens
Nerve Fibers
Intermediate Filament Proteins
Caveolae
Cells
Cultured

Connective Tissue Cells
education.field_of_study
Histocytochemistry
S100 Proteins
Mitochondria
Electrophysiology
Proto-Oncogene Proteins c-kit
Intercellular Junctions
medicine.anatomical_structure
symbols
Molecular Medicine
Female
Ubiquitin Thiolesterase
medicine.medical_specialty
Population
Biology
Caveolins
Article
Interstitial cell
symbols.namesake
Microscopy
Electron
Transmission

Telocyte
Chromogranins
medicine
Humans
education
Fallopian Tubes
Cell Nucleus
Lamina propria
Mucous Membrane
Staining and Labeling
Muscle
Smooth

Cell Biology
Actins
Interstitial cell of Cajal
Microscopy
Fluorescence

Ultrastructure
biology.protein
Blood Vessels
Chromogranin A
Cell Surface Extensions
Zdroj: Journal of Cellular and Molecular Medicine. 9:479-523
ISSN: 1582-4934
1582-1838
DOI: 10.1111/j.1582-4934.2005.tb00376.x
Popis: We describe here--presumably for the first time--a Cajal-like type of tubal interstitial cells (t-ICC), resembling the archetypal enteric ICC. t-ICC were demonstrated in situ and in vitro on fresh preparations (tissue cryosections and primary cell cultures) using methylene-blue, crystal-violet, Janus-Green B or MitoTracker-Green FM Probe vital stainings. Also, t-ICC were identified in fixed specimens by light microscopy (methylene-blue, Giemsa, trichrome stainings, Gomori silver-impregnation) or transmission electron microscopy (TEM). The positive diagnosis of t-ICC was strengthened by immunohistochemistry (IHC; CD117/c-kit+ and other 14 antigens) and immunofluorescence (IF; CD117/c-kit+ and other 7 antigens). The spatial density of t-ICC (ampullar-segment cryosections) was 100-150 cells/mm2. Non-conventional light microscopy (NCLM) of Epon semithin-sections revealed a network-like distribution of t-ICC in lamina propria and smooth muscle meshwork. t-ICC appeared located beneath of epithelium, in a 10-15 microm thick 'belt', where 18+/-2% of cells were t-ICC. In the whole lamina propria, t-ICC were about 9%, and in muscularis approximately 7%. In toto, t-ICC represent ~8% of subepithelial cells, as counted by NCLM. In vitro, t-ICC were 9.9+/-0.9% of total cell population. TEM showed that the diagnostic 'gold standard' (Huizinga et al., 1997) is fulfilled by 'our' t-ICC. However, we suggest a 'platinum standard', adding a new defining criterion- characteristic cytoplasmic processes (number: 1-5; length: tens of microm; thickness: < or =0.5 microm; aspect: moniliform; branching: dichotomous; organization: network, labyrinthic-system). Quantitatively, the ultrastructural architecture of t-ICC is: nucleus, 23.6+/-3.2% of cell volume, with heterochromatin 49.1+/-3.8%; mitochondria, 4.8+/-1.7%; rough and smooth endoplasmic-reticulum (1.1+/-0.6%, 1.0+/-0.2%, respectively); caveolae, 3.4+/-0.5%. We found more caveolae on the surface of cell processes versus cell body, as confirmed by IF for caveolins. Occasionally, the so-called 'Ca2+-release units' (subplasmalemmal close associations of caveolae+endoplasmic reticulum+mitochondria) were detected in the dilations of cell processes. Electrophysiological single unit recordings of t-ICC in primary cultures indicated sustained spontaneous electrical activity (amplitude of membrane potentials: 57.26+/-6.56 mV). Besides the CD117/c-kit marker, t-ICC expressed variously CD34, caveolins 1&2, alpha-SMA, S-100, vimentin, nestin, desmin, NK-1. t-ICC were negative for: CD68, CD1a, CD62P, NSE, GFAP, chromogranin-A, PGP9.5, but IHC showed the possible existence of (neuro)endocrine cells in tubal interstitium. We call them 'JF cells'. In conclusion, the identification of t-ICC might open the door for understanding some tubal functions, e.g. pace-making/peristaltism, secretion (auto-, juxta- and/or paracrine), regulation of neurotransmission (nitrergic/purinergic) and intercellular signaling, via the very long processes. Furthermore, t-ICC might even be uncommitted bipotential progenitor cells.
Databáze: OpenAIRE