A standalone approach to utilize telomere length measurement as a surveillance tool in oral leukoplakia
| Autor: | Shruti Shrivastava, Vivek Choudhary, Sovna Shivani Mishra, Pradeep K. Patra, Tripti Barardiya, Hansa Banjara, Yogita Rajput, Jagannath Pal, Pinaka Pani Ramakrishna, Masood A. Shammas, Renuka Gahine, Ankur Chandrakar, Varsha Mungutwar |
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| Rok vydání: | 2022 |
| Předmět: |
Male
Cancer Research medicine.medical_specialty High variability Peripheral blood mononuclear cell Gastroenterology External reference Internal medicine Genetics Humans Medicine business.industry General Medicine Venous blood Telomere medicine.disease Oral tissue Oral leukoplakia Oncology Head and Neck Neoplasms Dysplasia Carcinoma Squamous Cell Leukocytes Mononuclear Molecular Medicine Female Mouth Neoplasms Leukoplakia Oral business |
| Zdroj: | Molecular Oncology. 16:1650-1660 |
| ISSN: | 1878-0261 1574-7891 |
| DOI: | 10.1002/1878-0261.13133 |
| Popis: | Oral squamous cell carcinoma (OSCC) is often preceded by a white patch on a surface of the mouth, called oral leukoplakia (OL). As accelerated telomere length (TL) shortening in dividing epithelial cells may lead to oncogenic transformation, telomere length measurement could serve as a predictive biomarker in OL. However, due to high variability and lack of a universal reference, there has been a limited translational application. Here, we describe an approach of evaluating TL using paired peripheral blood mononuclear cells (PBMC) as an internal reference and demonstrate its translational relevance. Oral brush biopsy and paired venous blood were collected from 50 male OL patients and 44 male healthy controls (HC). Relative TL was measured by quantitative PCR. TL of each OL or healthy sample was normalized to the paired PBMC sample (TL ratio). In OL patients, the mean TL ratio was significantly smaller not only in the patch but also in distal normal oral tissue, relative to healthy controls without a high-risk oral habit. Dysplasia was frequently associated with a subgroup that showed a normal TL ratio at the patch but significantly smaller TL ratio at a paired normal distal site. Our data suggest that evaluation of TL attrition using a paired PBMC sample eliminates the requirement of external reference DNA, makes data universally comparable and provides a useful marker to define high-risk OL groups for follow-up programs. Larger studies will further validate the approach and its broader application in other premalignant conditions. |
| Databáze: | OpenAIRE |
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