Challenges and recommendations in developing LC–MS/MS bioanalytical assays of labile glucuronides and parent compounds in the presence of glucuronide metabolites
| Autor: | Qin C Ji, Xiaohui Xu, Long Yuan |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
Bioanalysis
Clinical Biochemistry Acyl glucuronide 030226 pharmacology & pharmacy 01 natural sciences Analytical Chemistry 03 medical and health sciences Glucuronides 0302 clinical medicine Tandem Mass Spectrometry In vivo Lc ms ms Humans General Pharmacology Toxicology and Pharmaceutics Chromatography Chemistry Hydrolysis 010401 analytical chemistry General Medicine Hydrogen-Ion Concentration In vitro 0104 chemical sciences Medical Laboratory Technology Biological Assay Glucuronide Chromatography Liquid |
| Zdroj: | Bioanalysis. 12:615-624 |
| ISSN: | 1757-6199 1757-6180 |
| DOI: | 10.4155/bio-2020-0055 |
| Popis: | Glucuronides, especially acyl glucuronides, were often found to be unstable in vitro and in vivo. Acyl glucuronide metabolites can convert back to the parent drugs at physiological pH through hydrolysis. Glucuronides can also undergo in-source fragmentation during MS ionization to form the same ions as those of the parent compounds, which could cause interference to the analysis of the parent compounds. All of these may cause significant challenges in developing LC–MS/MS bioanalytical assays of labile glucuronides or parent compounds in the presence of glucuronide metabolites. In this manuscript, we will discuss these challenges and summarize recommended strategies and practices for fast and efficient method development. Critical considerations in assay development will also be discussed. |
| Databáze: | OpenAIRE |
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