Efficacy of Massachusetts and 793B Vaccines Against Infectious Bronchitis Moroccan-Italy 02 Virus in Specific-Pathogen-Free Chickens and Commercial Broilers
| Autor: | Ouafaa Fassi Fihri, Mattias Delpont, Mariette F. Ducatez, Faouzi Kichou, Jean-Luc Guérin, Céline Bleuart, Marie-Noëlle Lucas, Sajid Umar, Sakhia F Z Belkasmi, Saâdia Nassik, Siham Fellahi, Maxence Delverdier, Mohammed El Houadfi |
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| Přispěvatelé: | Unité de Pathologie Aviaire, Département de Pathologie et Santé Publique Vétérinaire, Institut Agronomique et Vétérinaire Hassan II - IAV (MOROCCO) (IAV), Interactions hôtes-agents pathogènes [Toulouse] (IHAP), Institut National de la Recherche Agronomique (INRA)-Ecole Nationale Vétérinaire de Toulouse (ENVT), Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées, Ecole Nationale Vétérinaire de Toulouse (ENVT) |
| Rok vydání: | 2017 |
| Předmět: |
infectious bronchitis virus
0301 basic medicine Veterinary medicine 040301 veterinary sciences Infectious bronchitis virus Biology Serology 0403 veterinary science 03 medical and health sciences Food Animals medicine Animals Viral shedding commercial broiler Poultry Diseases Specific-pathogen-free specific-pathogen-free chicken [SDV.BA.MVSA]Life Sciences [q-bio]/Animal biology/Veterinary medicine and animal Health General Immunology and Microbiology [SDV.BA]Life Sciences [q-bio]/Animal biology Broiler Antibody titer Viral Vaccines 04 agricultural and veterinary sciences medicine.disease Avian infectious bronchitis biology.organism_classification Specific Pathogen-Free Organisms Virus Shedding 3. Good health 030104 developmental biology Massachusetts 793B vaccine [SDV.MP.VIR]Life Sciences [q-bio]/Microbiology and Parasitology/Virology Bronchitis Animal Science and Zoology Coronavirus Infections Moroccan-Italy 02 virus Chickens |
| Zdroj: | Avian diseases Avian diseases, American Association of Avian Pathologists, 2017, 61 (4), pp.466-471. ⟨10.1637/11686-060817-Reg.1⟩ |
| ISSN: | 1938-4351 0005-2086 |
| DOI: | 10.1637/11686-060817-reg.1 |
| Popis: | International audience; The ability of commercial vaccines H120 and 4/91 to protect against Moroccan-Italy 02 infectious bronchitis virus (Mor-It02) was investigated in specific-pathogen-free (SPF) chickens and commercial broiler chickens. Commercial broiler chicks (Experiment 1) were vaccinated at the hatchery with H120 vaccine at Day 1, and challenged at Day 21 with 104 50% egg-infective dose (EID50) of Mor-It02. All chicks were observed daily for clinical signs attributable to Mor-It02 infection during the 10 days postchallenge (pc). At 5 and 10 days pc, chicks were humanely sacrificed for necropsy examination, and tissues were collected for histopathology evaluation. To better understand the findings on commercial broilers, day-old SPF chicks were divided into five groups in a second experiment: Group Mass/4-91, vaccinated with H120 and 4/91 respectively at Days 1 and 15 of age; Group Mass/Mass, vaccinated by H120 at Days 1 and 15; Group Mass, vaccinated with H120 at Day 1; Group NV, kept unvaccinated; and Group NC, kept as a negative control (unchallenged). At Day 24 of age, Groups Mass/4-91, Mass/Mass, Mass, and NV were challenged with 104 EID50 of Mor-It02. In both experiments, blood samples were collected at different periods for serologic analyses. Oropharyngeal swabs were collected for virus detection by reverse-transcription PCR. In Experiments 1 and 2, respiratory signs started as early as 24 hr pc and maximum severity was observed on Days 3 and 4 pc. The viral shedding rate was significantly lower in Group Mass/4-91 compared to other challenged groups. Serologic analysis in both experiments showed that the sera of challenged group exhibited significantly higher antibody titers than sera collected before challenge. Histopathologic investigations in SPF birds showed deciliation and hyperplasia in Group NV and less-pronounced lesions in Groups Mass/Mass and Mass. In commercial broilers vaccinated with H120 alone, hyperplasia and deciliation were observed in 90% of the tracheas. These experiments illustrated that Mor-It02 is pathogenic for chickens and a combination of live H120 and 4/91 vaccines given respectively at Day 1 and Day 15 of age confer a good protection against Mor-It02. |
| Databáze: | OpenAIRE |
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