The DG75 B-cell lymphoma line exhibits biclonal immunoglobulin gene rearrangement
| Autor: | Jin-Wei Gao, Guanghua Wang, Jun Hu, Qiaoxia Hu, Yuan Li, Zongli Qi, Hua Guo, Xiangrong Zhao |
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| Rok vydání: | 2012 |
| Předmět: |
biology
General Neuroscience T-cell receptor Clone (cell biology) General Medicine Gene rearrangement Articles Cell cycle medicine.disease Molecular biology General Biochemistry Genetics and Molecular Biology medicine biology.protein Cancer research General Pharmacology Toxicology and Pharmaceutics Antibody B-cell lymphoma Immunoglobulin Gene Rearrangement Heteroduplex |
| Zdroj: | Biomedical reports. 1(1) |
| ISSN: | 2049-9434 |
| Popis: | Immunoglobulin (Ig) and T-cell receptor (TCR) gene rearrangement (GR) studies have been successfully employed to investigate the clonality and cell lineage of various lymphoid malignancies. Several lymphoma cell lines, such as BJAB, RAJI, DG75 and Jurkat cell lines, were often used as the positive controls in GR detection assays. Of those, the DG75 B-cell lymphoma line was found to exhibit biclonality [two or more homoduplex and heteroduplex bands in a polymerase chain reaction (PCR) product of clonality assay] in the PCR of GR detection assays. To further explore these characteristics of the biclonal phenomenon, the PCR products were purified and cloned into a pEGM-T clone vector. The sequences were analyzed using DNA analysis software. The results demonstrated that the two bands originated from two forms of GR of DG75 cell lines, i.e., DG75 is a biclonal cell line in Ig GRs, which has not been reported before. |
| Databáze: | OpenAIRE |
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