Fermented Rice Bran Supplementation Prevents the Development of Intestinal Fibrosis Due to DSS-Induced Inflammation in Mice
| Autor: | Tomonori Nochi, Kouichi Watanabe, Afifah Zahra Agista, Ardiansyah, Slamet Budijanto, Takuya Koseki, Halima Sultana, Hisashi Aso, Suh Ching Yang, Hitoshi Shirakawa, Jahidul Islam, Ryouta Hirakawa, Tubagus Bahtiar Rusbana, Yusuke Ohsaki, Michio Komai |
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| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
0301 basic medicine
colitis medicine.medical_treatment Mice 0302 clinical medicine Fibrosis intestinal inflammation TX341-641 education.field_of_study Nutrition and Dietetics Tight junction digestive oral and skin physiology Dextran Sulfate food and beverages Cytokine 030211 gastroenterology & hepatology Female medicine.symptom fermented rice bran medicine.medical_specialty Population Inflammation Article 03 medical and health sciences Internal medicine medicine intestinal fibrosis Animals Colitis education Bran business.industry Nutrition. Foods and food supply Oryza dextran sodium sulfate medicine.disease digestive system diseases Diet carbohydrates (lipids) Mice Inbred C57BL stomatognathic diseases Disease Models Animal Intestinal Diseases 030104 developmental biology Endocrinology Dietary Supplements Fermentation business Food Science |
| Zdroj: | Nutrients, Vol 13, Iss 1869, p 1869 (2021) Nutrients Volume 13 Issue 6 |
| ISSN: | 2072-6643 |
| Popis: | Fermented rice bran (FRB) is known to protect mice intestines against dextran sodium sulfate (DSS)-induced inflammation however, the restoration of post-colitis intestinal homeostasis using FRB supplementation is currently undocumented. In this study, we observed the effects of dietary FRB supplementation on intestinal restoration and the development of fibrosis after DSS-induced colitis. DSS (1.5%) was introduced in the drinking water of mice for 5 days. Eight mice were sacrificed immediately after the DSS treatment ended. The remaining mice were divided into three groups, comprising the following diets: control, 10% rice bran (RB), and 10% FRB-supplemented. Diet treatment was continued for 2 weeks, after which half the population of mice from each group was sacrificed. The experiment was continued for another 3 weeks before the remaining mice were sacrificed. FRB supplementation could reduce the general observation of colitis and production of intestinal pro-inflammatory cytokines. FRB also increased intestinal mRNA levels of anti-inflammatory cytokine, tight junction, and anti-microbial proteins. Furthermore, FRB supplementation suppressed markers of intestinal fibrosis. This effect might have been achieved via the canonical Smad2/3 activation and the non-canonical pathway of Tgf-β activity. These results suggest that FRB may be an alternative therapeutic agent against inflammation-induced intestinal fibrosis. |
| Databáze: | OpenAIRE |
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