Combinatorial peptide synthesis on a microchip
| Autor: | Gloria Torralba, Frank Breitling, Kai König, Simon Fernandez, Ines Block, Volker Stadler, Klaus Leibe, F. Ralf Bischoff, Thomas Felgenhauer, Alexander Nesterov, Michael Hausmann, Christopher Schirwitz, Volker Lindenstruth |
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| Rok vydání: | 2009 |
| Předmět: |
Polymers
Surface Properties Protein Array Analysis Peptide Biochemistry Polyethylene Glycols chemistry.chemical_compound Coated Materials Biocompatible Structural Biology Peptide synthesis Combinatorial Chemistry Techniques Amino Acids Particle Size chemistry.chemical_classification Atom-transfer radical-polymerization Proteins Combinatorial chemistry Amino acid chemistry Graft polymer Surface modification Methacrylates Adsorption Peptides Ethylene glycol Protein adsorption |
| Zdroj: | Current Protocols in Protein Science |
| ISSN: | 1934-3663 |
| Popis: | Microchips are used in the combinatorial synthesis of peptide arrays by means of amino acid microparticle deposition. The surface of custom-built microchips can be equipped with an amino-modified poly(ethylene glycol)methacrylate (PEGMA) graft polymer coating, which permits high loading of functional groups and resists nonspecific protein adsorption. Specific microparticles that are addressed to the polymer-coated microchip surface in a well defined pattern release preactivated amino acids upon melting, and thus allow combinatorial synthesis of high-complexity peptide arrays directly on the chip surface. Currently, arrays with densities of up to 40,000 peptide spots/cm2 can be generated in this way, with a minimum of coupling cycles required for full combinatorial synthesis. Without using any additional blocking agent, specific peptide recognition has been verified by background-free immunostaining on the chip-based array. This unit describes microchip surface modification, combinatorial peptide array synthesis on the chip, and a typical immunoassay employing the resulting high-density peptide arrays. Curr. Protoc. Protein Sci. 57:18.2.1-18.2.13. © 2009 by John Wiley & Sons, Inc. Keywords: solid phase peptide synthesis; peptide array; combinatorial synthesis; atom transfer radical polymerization (ATRP); amino acid particles |
| Databáze: | OpenAIRE |
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