Assessment of the in vivo biofunctionality of a biomimetic hybrid scaffold for osteochondral tissue regeneration
| Autor: | Serena Bertoldi, Matilde Tschon, Silvia Brogini, Ornella Parolini, Annapaola Parrilli, Lucia Martini, Antonietta Silini, Milena Fini, Silvia Farè, Francesca Veronesi, Gianluca Giavaresi |
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| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
0106 biological sciences
0301 basic medicine Cartilage Articular Male Scaffold Bone Regeneration osteochondral regeneration Bioengineering Mesenchymal Stem Cell Transplantation 01 natural sciences Applied Microbiology and Biotechnology calcium phosphate 03 medical and health sciences Tissue engineering In vivo Biomimetic Materials 010608 biotechnology Highly porous medicine Animals Humans Settore BIO/13 - BIOLOGIA APPLICATA Femur Bone regeneration pectin Tissue Scaffolds Chemistry Cartilage Mesenchymal stem cell Mesenchymal Stem Cells human amniotic mesenchymal stromal cells Cells Immobilized polyurethane scaffold 030104 developmental biology medicine.anatomical_structure Heterografts Implant Rabbits Biotechnology Biomedical engineering |
| Popis: | Chondral and osteochondral lesions represent one of the most challenging problems in the orthopedic field, as these types of injuries lead to disability and worsened quality of life for patients and have an economic impact on the healthcare system. The aim of this in vivo study was to develop a new tissue engineering approach through a hybrid scaffold for osteochondral tissue regeneration made of porous polyurethane foam (PU) coated under vacuum with calcium phosphates (PU/VAC). Scaffold characterization showed a highly porous and interconnected structure. Human amniotic mesenchymal stromal cells (hAMSCs) were loaded into scaffolds using pectin (PECT) as a carrier. Osteochondral defects in medial femoral condyles of rabbits were created and randomly allocated in one of the following groups: plain scaffold (PU/VAC), scaffold with hAMSCs injected in the implant site (PU/VAC/hAMSC), scaffold with hAMSCs loaded in pectin (PU/VAC/PECT/hAMSC), and no treated defects (untreated). The therapeutic efficacy was assessed by macroscopic, histological, histomorphometric, microtomographic, and ultrastructural analyses at 3, 6, 12, and 24 weeks. Histological results showed that the scaffold was permissive to tissue growth and penetration, an immature osteocartilaginous tissue was observed at early experimental times, with a more accentuated bone regeneration in comparison with the cartilage layer in the absence of any inflammatory reaction. |
| Databáze: | OpenAIRE |
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