Arginine supplementation induces myoblast fusion via augmentation of nitric oxide production
| Autor: | Jenna L. Betters, Quinlyn A. Soltow, Vitor A. Lira, David S. Criswell, Jodi H. D. Long, Jeff E. Sellman |
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| Rok vydání: | 2006 |
| Předmět: |
Myoblast proliferation
Time Factors Arginine Physiology Myoblasts Skeletal TRPV Cation Channels Endogeny Nitric Oxide Biochemistry Membrane Fusion Nitric oxide Cell Line Myoblast fusion chemistry.chemical_compound Mice Myocyte Animals RNA Messenger Nitrites Nitrates biology Chemistry Myogenesis Histocytochemistry Cell Differentiation Cell Biology Cell biology Nitric oxide synthase NG-Nitroarginine Methyl Ester biology.protein Calcium Channels |
| Zdroj: | Journal of muscle research and cell motility. 27(8) |
| ISSN: | 0142-4319 |
| Popis: | The semi-essential amino acid, L-arginine (L-Arg), is the substrate for endogenous synthesis of nitric oxide, a molecule that is involved in myoblast proliferation and fusion. Since L-Arg supply may limit nitric oxide synthase (NOS) activity in endothelial cells, we examined L-Arg supplementation in differentiating mouse myoblasts and tested the hypothesis that L-Arg exerts direct effects on myoblast fusion via augmentation of endogenous nitric oxide production. C(2)C(12) myoblasts in differentiation media received one of the following treatments for 120 h: 1 mM L-Arg, 0.1 mM N-nitro-L-arginine methyl ester (L-NAME), L-Arg + L-NAME, 10 mM L-Lysine, or no supplement (Control). Cultures were fixed and stained with hematoxylin and eosin for microphotometric image analysis of myotube density, nuclear density, and fusion index (% of total nuclei in myotubes). Endogenous production of nitric oxide during the treatment period peaked between 24 and 48 h. L-Arg amplified nitric oxide production between 0 and 24 h and increased myotube density, total nuclei number, and nuclear fusion index. These L-Arg effects were prevented by the NOS inhibitor, L-NAME. Further, L-Lysine, a competitive inhibitor of L-Arg uptake, repressed nitric oxide production and reduced myotube density and fusion index. In summary, L-Arg augments myotube formation and increases nitric oxide production in a process limited by cellular L-Arg uptake. |
| Databáze: | OpenAIRE |
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