Elimination of ESAT-6 and CFP-10 from a candidate vaccine against bovine tuberculosis impaired its protection efficacy in the BALBc mouse model
| Autor: | Maria Emilia Eirin, Elizabeth Andrea Garcia, Laura Inés Klepp, Fabiana Bigi, Federico Carlos Blanco, Ximena Ferrara Muñiz |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
Microbiology (medical)
Tuberculosis 030231 tropical medicine lcsh:QR1-502 complex mixtures lcsh:Microbiology cfp-10 03 medical and health sciences Mice 0302 clinical medicine Immune system Antigen Bacterial Proteins vaccine medicine Animals 030212 general & internal medicine bovine tuberculosis esat-6 Mycobacterium bovis Antigens Bacterial Vaccines CFP-10 biology medicine.disease biology.organism_classification bacterial infections and mycoses Virology Diva Vaccination mycobacterium bovis Infectious Diseases ESAT-6 Cattle Tuberculosis Bovine |
| Zdroj: | International Journal of Mycobacteriology, Vol 9, Iss 4, Pp 417-421 (2020) |
| ISSN: | 2212-5531 |
| Popis: | Background Bovine tuberculosis (bTB) is a zoonotic disease caused by Mycobacterium bovis that mainly affects cattle. Although vaccination is the most effective strategy to control bTB, it may interfere with the diagnosis of the infection. Therefore, ancillary tests to differentiate vaccinated from infected animals (DIVA) are essential in a cattle vaccination scenario. ESAT-6 and CFP-10 are the most promissory DIVA antigens. Method In this study, we deleted esat6 and cfp10 genes from the M. bovis Δ mce2 live-attenuated vaccine candidate and evaluated its protection level against bTB in BALBc mice. Results We found that the M. bovis strain mutant in mce2, esat-6 and cfp-10 failed to confer protection against virulent M. bovis challenge in a mouse model of tuberculosis. Conclusions This result highlights the relevant role of ESAT-6 and CFP-10 in the induction of protective immune response against M. bovis infection and reveals the need of evaluating different strategies to compensate for the lack of these DIVA antigens in new vaccine formulations. |
| Databáze: | OpenAIRE |
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