Interallelic Transcriptional Enhancement as an in Vivo Measure of Transvection in Drosophila melanogaster
| Autor: | Geoffrey P. Noble, Surachai Supattapone, Patrick J. Dolph |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
GAL4/UAS system Candidate gene Transcription Genetic somatic pairing Gene Expression Investigations QH426-470 Prion Proteins Animals Genetically Modified 03 medical and health sciences Gene expression Genetics Animals Transgenes RNA Small Interfering Enhancer Molecular Biology Alleles Genetics (clinical) Transvection Regulation of gene expression biology transvection Epistasis Genetic biology.organism_classification Drosophila melanogaster Enhancer Elements Genetic 030104 developmental biology Gene Expression Regulation Gene Knockdown Techniques GAL4-UAS Trans-acting enhancer |
| Zdroj: | G3: Genes, Genomes, Genetics, Vol 6, Iss 10, Pp 3139-3148 (2016) G3: Genes|Genomes|Genetics |
| ISSN: | 2160-1836 |
| Popis: | Transvection—pairing-dependent interallelic regulation resulting from enhancer action in trans—occurs throughout the Drosophila melanogaster genome, likely as a result of the extensive somatic homolog pairing seen in Dipteran species. Recent studies of transvection in Drosophila have demonstrated important qualitative differences between enhancer action in cisvs.in trans, as well as a modest synergistic effect of cis- and trans-acting enhancers on total tissue transcript levels at a given locus. In the present study, we identify a system in which cis- and trans-acting GAL4-UAS enhancer synergism has an unexpectedly large quantitative influence on gene expression, boosting total tissue transcript levels at least fourfold relative to those seen in the absence of transvection. We exploit this strong quantitative effect by using publicly available UAS-shRNA constructs from the TRiP library to assay candidate genes for transvection activity in vivo. The results of the present study, which demonstrate that in trans activation by simple UAS enhancers can have large quantitative effects on gene expression in Drosophila, have important new implications for experimental design utilizing the GAL4-UAS system. |
| Databáze: | OpenAIRE |
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