The microRNA miR-33 is a pleiotropic regulator of metabolic and developmental processes in Drosophila melanogaster
| Autor: | Guido T. Bommer, Laure-Alix Clerbaux, Dan Fu Ruan, Jennifer A. Kennell, Ronald Yu, Abigail M. Lamb, Hayley Schultz, Samara Roman-Holba |
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| Rok vydání: | 2021 |
| Předmět: |
0301 basic medicine
miR-33 Mammals biology Mutant Regulator Lipid metabolism biology.organism_classification Lipid Metabolism Phenotype Cell biology 03 medical and health sciences MicroRNAs 030104 developmental biology 0302 clinical medicine Drosophila melanogaster Cuticle pigmentation Animals Drosophila Proteins Gene 030217 neurology & neurosurgery Triglycerides Developmental Biology |
| Zdroj: | Developmental dynamics : an official publication of the American Association of AnatomistsREFERENCES. 250(11) |
| ISSN: | 1097-0177 |
| Popis: | Background miR-33 family members are well characterized regulators of cellular lipid levels in mammals. Previous studies have shown that overexpression of miR-33 in Drosophila melanogaster leads to elevated triacylglycerol (TAG) levels in certain contexts. Although loss of miR-33 in flies causes subtle defects in larval and adult ovaries, the effects of miR-33 deficiency on lipid metabolism and other phenotypes impacted by metabolic state have not yet been characterized. Results We found that loss of miR-33 predisposes flies to elevated TAG levels, and we identified genes involved in TAG synthesis as direct targets of miR-33, including atpcl, midway, and Akt1. miR-33 mutants survived longer upon starvation but showed greater sensitivity to an oxidative stressor. We also found evidence that miR-33 is a negative regulator of cuticle pigmentation and that miR-33 mutants show a reduction in interfollicular stalk cells during oogenesis. Conclusion Our data suggest that miR-33 is a conserved regulator of lipid homeostasis, and its targets are involved in both degradation and synthesis of fatty acids and TAG. The constellation of phenotypes involving tissues that are highly sensitive to metabolic state suggests that miR-33 serves to prevent extreme fluctuations in metabolically sensitive tissues. This article is protected by copyright. All rights reserved. |
| Databáze: | OpenAIRE |
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