Human Gingival Fibroblast and Osteoblast Behavior on Groove-Milled Zirconia Implant Surfaces

Autor: Brito da Cruz, Mariana, Marques, Joana, Silva, Neusa, Madeira, Sara, Carvalho, Óscar, Silva, Filipe Samuel, Caramês, João, Mata, António
Přispěvatelé: Repositório da Universidade de Lisboa, Universidade do Minho
Rok vydání: 2022
Předmět:
Zdroj: Materials; Volume 15; Issue 7; Pages: 2481
ISSN: 1996-1944
DOI: 10.3390/ma15072481
Popis: © 2022 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
Two type of cells representing periodontal hard tissues (osteoblasts) and soft tissues (fibroblasts) were evaluated in response to microgroove-milled zirconia surfaces. A total of 90 zirconia discs were randomly assigned to four width-standardized milling microgroove-textured groups and a control group without grooves (UT). The sandblast and acid-etch protocol were applied to all samples. Both cell lines were cultured on zirconia discs from 1 day up to 14 days. Cell morphology and adhesion were evaluated after 1 day of culturing. Cell viability and proliferation of the cells were measured. Alkaline phosphatase activity, collagen I, osteopontin, interleukin 1β and interleukin 8 secretions were assessed at predefined times. The results obtained were presented in the form of bar graphs as means and standard deviations. Multi comparisons between groups were evaluated using two-away ANOVA or Mann-Whitney tests, and a p-value < 0.05 was established. Group comparisons with regard to cell viability, proliferation and secretion of collagen I, interleukin-1β and interleukin 8 revealed no statistically significant differences. The alkaline phosphatase activity and osteopontin secretion were significantly higher in the group with a large groove compared to the small one and the control group. Nevertheless, the viability of gingival and bone cells did not appear to be affected by the milled microgroove texture compared to the conventional sandblasted and acid-etched texture, but they seem to influence osteoblasts' cellular differentiation.
This research was supported by FCT project POCI-01-0145-FEDER-030498—Portugal, by FEDER funds through the COMPETE 2020—Programa Operacional Competitividade e Internacionalização (POCI) and ImpHib–Development of advanced Hybrid Implants—NORTE-01-0247-FEDER-034074.
Databáze: OpenAIRE
Externí odkaz:
Nepřihlášeným uživatelům se plný text nezobrazuje